BD PhosflowTM RB780 Mouse Anti-Stat1 (pY701)

Description

Flow cytometric analysis of Stat1 pY701 expression in Unstimulated and Stimulated Human CD4+ T lymphocytes. Human peripheral blood leucocytes were either not stimulated Unstimulated, dashed line histogram or stimulated Stimulated, solid line histogram for 15 minutes with Phorbol 12-Myristate 13-Acetate Sigma P-8139 as well as Recombinant Human IFN-alpha, IL-2, IL-4, and IL-6 proteins. The cells were harvested, washed with BD Pharmingen Stain Buffer FBS Cat. No. 554656 , fixed with BD Cytofix Fixation Buffer Cat. No. 554655 , and then permeabilized with BD Phosflow Perm Buffer III Cat. No. 558050 . After washing and resuspending in Stain Buffer, the cells were stained with APC Mouse Anti-Human CD4 antibody Cat. No. 561841 and BD Phosflow RB780 Mouse Anti-Stat1 pY701 antibody. The fluorescence histograms showing Stat1 pY701 expression were derived from gated CD4+ T cells with the forward and side light-scatter characteristics of intact lymphocytes. Flow cytometry and data analysis were performed using a BD LSR II Flow Cytometry System and FlowJo software. Flow cytometric analysis of Stat1 pY701 expression in Unstimulated and Stimulated Human CD4+ T lymphocytes. Human peripheral blood leucocytes were either not stimulated Unstimulated, dashed line histogram or stimulated Stimulated, solid line histogram for 15 minutes with Phorbol 12-Myristate 13-Acetate Sigma P-8139 as well as Recombinant Human IFN-alpha, IL-2, IL-4, and IL-6 proteins. The cells were harvested, washed with BD Pharmingen Stain Buffer FBS Cat. No. 554656 , fixed with BD Cytofix Fixation Buffer Cat. No. 554655 , and then permeabilized with BD Phosflow Perm Buffer III Cat. No. 558050 . After washing and resuspending in Stain Buffer, the cells were stained with APC Mouse Anti-Human CD4 antibody Cat. No. 561841 and BD Phosflow RB780 Mouse Anti-Stat1 pY701 antibody. The fluorescence histograms showing Stat1 pY701 expression were derived from gated CD4+ T cells with the forward and side light-scatter characteristics of intact lymphocytes. Flow cytometry and data analysis were performed using a BD LSR II Flow Cytometry System and FlowJo software. Show More Flow cytometric analysis of Stat1 pY701 expression in Unstimulated and Stimulated Human CD4+ T lymphocytes. Human peripheral blood leucocytes were either not stimulated Unstimulated, dashed line histogram or stimulated Stimulated, solid line histogram for 15 minutes with Phorbol 12-Myristate 13-Acetate Sigma P-8139 as well as Recombinant Human IFN-alpha, IL-2, IL-4, and IL-6 proteins. The cells were harvested, washed with BD Pharmingen Stain Buffer FBS Cat. No. 554656 , fixed with BD Cytofix Fixation Buffer Cat. No. 554655 , and then permeabilized with BD Phosflow Perm Buffer III Cat. No. 558050 . After washing and resuspending in Stain Buffer, the cells were stained with APC Mouse Anti-Human CD4 antibody Cat. No. 561841 and BD Phosflow RB780 Mouse Anti-Stat1 pY701 antibody. The fluorescence histograms showing Stat1 pY701 expression were derived from gated CD4+ T cells with the forward and side light-scatter characteristics of intact lymphocytes. Flow cytometry and data analysis were performed using a BD LSR II Flow Cytometry System and FlowJo software.