BD PhosflowTM RY586 Mouse Anti-Stat1 (pY701)

Description

Two-color flow cytometric analysis of Stat1 pY701 expression in Human CD4+ T lymphocytes. Untreated dashed line histogram or Treated solid line histogram Human peripheral blood mononuclear cells PBMC from the BD Phosflow T Cell Kit Lyophilized Cells Catalog No. 560760 were reconstituted in BD Pharmingen Stain Buffer Cat. No. 554656 for 15 minutes at room temperature. These cells are fixed, permeabilized, and lyophilized Human PBMC that are either Untreated or Treated with stimulators including Phorbol 12-Myristate 13-Acetate PMA , and Recombinant Human Interferon-alpha IFN-alpha , Interleukin-2 IL-2 , IL-4, and IL-6 proteins . The reconstituted cells were then washed and stained with Alexa Fluor 488 Mouse Anti-Human CD4 antibody Cat. No. 557695 and BD Phosflow RY586 Mouse Anti-Stat1 pY701 antibody Cat. No. 568528/568529 . Histograms showing Stat1 pY701 expression were derived from CD4-positive gated events with the forward and side light-scatter characteristics of intact lymphocytes. Flow cytometry and data analysis were performed using a BD LSR II Flow Cytometry System and FlowJo software. Two-color flow cytometric analysis of Stat1 pY701 expression in Human CD4+ T lymphocytes. Untreated dashed line histogram or Treated solid line histogram Human peripheral blood mononuclear cells PBMC from the BD Phosflow T Cell Kit Lyophilized Cells Catalog No. 560760 were reconstituted in BD Pharmingen Stain Buffer Cat. No. 554656 for 15 minutes at room temperature. These cells are fixed, permeabilized, and lyophilized Human PBMC that are either Untreated or Treated with stimulators including Phorbol 12-Myristate 13-Acetate PMA , and Recombinant Human Interferon-alpha IFN-alpha , Interleukin-2 IL-2 , IL-4, and IL-6 proteins . The reconstituted cells were then washed and stained with Alexa Fluor 488 Mouse Anti-Human CD4 antibody Cat. No. 557695 and BD Phosflow RY586 Mouse Anti-Stat1 pY701 antibody Cat. No. 568528/568529 . Histograms showing Stat1 pY701 expression were derived from CD4-positive gated events with the forward and side light-scatter characteristics of intact lymphocytes. Flow cytometry and data analysis were performed using a BD LSR II Flow Cytometry System and FlowJo software. Show More Two-color flow cytometric analysis of Stat1 pY701 expression in Human CD4+ T lymphocytes. Untreated dashed line histogram or Treated solid line histogram Human peripheral blood mononuclear cells PBMC from the BD Phosflow T Cell Kit Lyophilized Cells Catalog No. 560760 were reconstituted in BD Pharmingen Stain Buffer Cat. No. 554656 for 15 minutes at room temperature. These cells are fixed, permeabilized, and lyophilized Human PBMC that are either Untreated or Treated with stimulators including Phorbol 12-Myristate 13-Acetate PMA , and Recombinant Human Interferon-alpha IFN-alpha , Interleukin-2 IL-2 , IL-4, and IL-6 proteins . The reconstituted cells were then washed and stained with Alexa Fluor 488 Mouse Anti-Human CD4 antibody Cat. No. 557695 and BD Phosflow RY586 Mouse Anti-Stat1 pY701 antibody Cat. No. 568528/568529 . Histograms showing Stat1 pY701 expression were derived from CD4-positive gated events with the forward and side light-scatter characteristics of intact lymphocytes. Flow cytometry and data analysis were performed using a BD LSR II Flow Cytometry System and FlowJo software.