BD PhosflowTM RY586 Mouse Anti-Stat5 (pY694)

Description

Flow cytometric analysis of Stat5 PY694 expression in human CD4+ T lymphocytes. Untreated dashed line histogram or treated solid line histogram peripheral blood mononuclear cells PBMC from the BD Phosflow T Cell Kit Lyophilized Cells Catalog No. 560760 were reconstituted in BD Pharmingen Stain Buffer Cat. No. 554656 for 15 minutes at room temperature. These cells were fixed, permeabilized, and lyophilized PBMC that were either untreated or treated with stimulators including phorbol 12-myristate 13-acetate PMA , interferon-alpha IFN-alpha , interleukin-2 IL-2 , IL-4, and IL-6 . The reconstituted cells were then washed and stained with BD Pharmingen APC Mouse Anti-Human CD4 antibody Cat. No. 566915 and BD Phosflow RY586 Mouse Anti-Human Stat5 pY694 antibody Cat. No. 568145/568146 . Histograms showing Stat5 pY694 expression were derived from CD4-positive gated events with the forward and side light-scatter characteristics of intact lymphocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa Flow Cytometer System and FlowJo software. Flow cytometric analysis of Stat5 PY694 expression in human CD4+ T lymphocytes. Untreated dashed line histogram or treated solid line histogram peripheral blood mononuclear cells PBMC from the BD Phosflow T Cell Kit Lyophilized Cells Catalog No. 560760 were reconstituted in BD Pharmingen Stain Buffer Cat. No. 554656 for 15 minutes at room temperature. These cells were fixed, permeabilized, and lyophilized PBMC that were either untreated or treated with stimulators including phorbol 12-myristate 13-acetate PMA , interferon-alpha IFN-alpha , interleukin-2 IL-2 , IL-4, and IL-6 . The reconstituted cells were then washed and stained with BD Pharmingen APC Mouse Anti-Human CD4 antibody Cat. No. 566915 and BD Phosflow RY586 Mouse Anti-Human Stat5 pY694 antibody Cat. No. 568145/568146 . Histograms showing Stat5 pY694 expression were derived from CD4-positive gated events with the forward and side light-scatter characteristics of intact lymphocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa Flow Cytometer System and FlowJo software. Show More Flow cytometric analysis of Stat5 PY694 expression in human CD4+ T lymphocytes. Untreated dashed line histogram or treated solid line histogram peripheral blood mononuclear cells PBMC from the BD Phosflow T Cell Kit Lyophilized Cells Catalog No. 560760 were reconstituted in BD Pharmingen Stain Buffer Cat. No. 554656 for 15 minutes at room temperature. These cells were fixed, permeabilized, and lyophilized PBMC that were either untreated or treated with stimulators including phorbol 12-myristate 13-acetate PMA , interferon-alpha IFN-alpha , interleukin-2 IL-2 , IL-4, and IL-6 . The reconstituted cells were then washed and stained with BD Pharmingen APC Mouse Anti-Human CD4 antibody Cat. No. 566915 and BD Phosflow RY586 Mouse Anti-Human Stat5 pY694 antibody Cat. No. 568145/568146 . Histograms showing Stat5 pY694 expression were derived from CD4-positive gated events with the forward and side light-scatter characteristics of intact lymphocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa Flow Cytometer System and FlowJo software.