BD PhosflowTM RY703 Mouse Anti-Stat1 (pY701)

Description

Flow cytometric analysis of Stat1 pY701 expression in Unstimulated and Stimulated Human peripheral blood monocytes. Human peripheral blood mononuclear cells PBMC were either left Unstimulated dashed line histogram or Stimulated solid line histogram with 100 ng/ml final concentration of BD Pharmingen Recombinant Human IFN-gamma protein Cat. No. 554617 for 15 minutes at 37C. The PBMC were fixed using BD Cytofix Fixation buffer Cat. No. 554655 for 10 minutes at 37C, then permeabilized using BD Phosflow Perm Buffer III Cat. No. 558050 on ice for 30 minutes. The cells were washed twice with stain buffer and then stained with BD Phosflow RY703 Mouse Anti-Stat1 pY701 antibody Cat. No. 571799/571850 . Flow cytometry and data analysis were performed using a 5-laser Flow Cytometer System and FlowJo Software. Flow cytometric analysis of Stat1 pY701 expression in Unstimulated and Stimulated Human peripheral blood monocytes. Human peripheral blood mononuclear cells PBMC were either left Unstimulated dashed line histogram or Stimulated solid line histogram with 100 ng/ml final concentration of BD Pharmingen Recombinant Human IFN-gamma protein Cat. No. 554617 for 15 minutes at 37C. The PBMC were fixed using BD Cytofix Fixation buffer Cat. No. 554655 for 10 minutes at 37C, then permeabilized using BD Phosflow Perm Buffer III Cat. No. 558050 on ice for 30 minutes. The cells were washed twice with stain buffer and then stained with BD Phosflow RY703 Mouse Anti-Stat1 pY701 antibody Cat. No. 571799/571850 . Flow cytometry and data analysis were performed using a 5-laser Flow Cytometer System and FlowJo Software. Show More Flow cytometric analysis of Stat1 pY701 expression in Unstimulated and Stimulated Human peripheral blood monocytes. Human peripheral blood mononuclear cells PBMC were either left Unstimulated dashed line histogram or Stimulated solid line histogram with 100 ng/ml final concentration of BD Pharmingen Recombinant Human IFN-gamma protein Cat. No. 554617 for 15 minutes at 37C. The PBMC were fixed using BD Cytofix Fixation buffer Cat. No. 554655 for 10 minutes at 37C, then permeabilized using BD Phosflow Perm Buffer III Cat. No. 558050 on ice for 30 minutes. The cells were washed twice with stain buffer and then stained with BD Phosflow RY703 Mouse Anti-Stat1 pY701 antibody Cat. No. 571799/571850 . Flow cytometry and data analysis were performed using a 5-laser Flow Cytometer System and FlowJo Software.