BD StemflowTM Human Definitive and Pancreatic Endoderm Analysis Kit

Description

Definitive Endoderm differentiation of H9 hESC: H9 human embryonic stem cells hESCs WiCell, Madison, WI upper panels and differentiated cells lower panels were analyzed by flow cytometry using components of the BD Stemflow Human Definitive and Pancreatic Endoderm Analysis Kit. H9 hESCs were cultured on irradiated mouse embryonic fibroblasts in hESC medium DMEM:F12, 20 % Knockout Serum Replacement KOSR , non-essential amino acids, 20 mM glutamine Life Technologies , 1X penicillin/streptomycin Lonza , bFGF Cat. No. 354060 . For definitive endoderm differentiation, hESCs were differentiated for three days in 0.5 % FBS, 20mM glutamine, RPMI Life Technologies and 100ng/ml Activin A R&D Systems DAmour et al., 2005 . Flow cytometry was performed on a BD LSR II flow cytometry system. Pancreatic endoderm differentiation of CyT49 hESCs : CyT49 Human embryonic stem cells hESCs and differentiated cells were analyzed by flow cytometry using components of the BD Stemflow Human Definitive and Pancreatic Endoderm Analysis Kit. CyT49 hESCs were cultured and differentiated as previously described DAmour et al., 2006; Kroon et al., 2008, and Kelley et al., 2011 . Flow cytometry was performed on a BD LSR II flow cytometry system. Data provided by ViaCyte, Inc. Definitive Endoderm differentiation of H9 hESC: H9 human embryonic stem cells hESCs WiCell, Madison, WI upper panels and differentiated cells lower panels were analyzed by flow cytometry using components of the BD Stemflow Human Definitive and Pancreatic Endoderm Analysis Kit. H9 hESCs were cultured on irradiated mouse embryonic fibroblasts in hESC medium DMEM:F12, 20 % Knockout Serum Replacement KOSR , non-essential amino acids, 20 mM glutamine Life Technologies , 1X penicillin/streptomycin Lonza , bFGF Cat. No. 354060 . For definitive endoderm differentiation, hESCs were differentiated for three days in 0.5 % FBS, 20mM glutamine, RPMI Life Technologies and 100ng/ml Activin A R&D Systems DAmour et al., 2005 . Flow cytometry was performed on a BD LSR II flow cytometry system. Show More Pancreatic endoderm differentiation of CyT49 hESCs : CyT49 Human embryonic stem cells hESCs and differentiated cells were analyzed by flow cytometry using components of the BD Stemflow Human Definitive and Pancreatic Endoderm Analysis Kit. CyT49 hESCs were cultured and differentiated as previously described DAmour et al., 2006; Kroon et al., 2008, and Kelley et al., 2011 . Flow cytometry was performed on a BD LSR II flow cytometry system. Data provided by ViaCyte, Inc. Show More Definitive Endoderm differentiation of H9 hESC: H9 human embryonic stem cells hESCs WiCell, Madison, WI upper panels and differentiated cells lower panels were analyzed by flow cytometry using components of the BD Stemflow Human Definitive and Pancreatic Endoderm Analysis Kit. H9 hESCs were cultured on irradiated mouse embryonic fibroblasts in hESC medium DMEM:F12, 20 % Knockout Serum Replacement KOSR , non-essential amino acids, 20 mM glutamine Life Technologies , 1X penicillin/streptomycin Lonza , bFGF Cat. No. 354060 . For definitive endoderm differentiation, hESCs were differentiated for three days in 0.5 % FBS, 20mM glutamine, RPMI Life Technologies and 100ng/ml Activin A R&D Systems DAmour et al., 2005 . Flow cytometry was performed on a BD LSR II flow cytometry system. Pancreatic endoderm differentiation of CyT49 hESCs : CyT49 Human embryonic stem cells hESCs and differentiated cells were analyzed by flow cytometry using components of the BD Stemflow Human Definitive and Pancreatic Endoderm Analysis Kit. CyT49 hESCs were cultured and differentiated as previously described DAmour et al., 2006; Kroon et al., 2008, and Kelley et al., 2011 . Flow cytometry was performed on a BD LSR II flow cytometry system. Data provided by ViaCyte, Inc.