BD Transduction LaboratoriesTM Purified Mouse Anti-Caveolin 1

Description

Left: Western blot analysis of Caveolin 1 on a human endothelial lysate. Lane 1: 1:500, lane 2: 1:1000, lane 3: 1:2000 dilution of the Caveolin 1 antibody. Right: Immunofluorescent staining of HeLa ATCC CCL-2 cells. Cells were seeded in a 96 well imaging plate Cat. No. 353219 at 10 000 cells per well. After overnight incubation, cells were stained using the Triton X-100 perm protocol and the anti-Caveolin 1 antibody. The second step reagent was FITC goat anti mouse Ig Cat. No. 554001 . Images were taken on a BD Pathway 855 bioimaging system using a 20x objective. This antibody also stained A549 ATCC CCL-185 cells and worked with both the Triton X-100 and alcohol fix/perm protocols see Recommended Assay Procedure . This antibody is not recommended for staining U-2 OS cells. Left: Western blot analysis of Caveolin 1 on a human endothelial lysate. Lane 1: 1:500, lane 2: 1:1000, lane 3: 1:2000 dilution of the Caveolin 1 antibody. Right: Immunofluorescent staining of HeLa ATCC CCL-2 cells. Cells were seeded in a 96 well imaging plate Cat. No. 353219 at 10 000 cells per well. After overnight incubation, cells were stained using the Triton X-100 perm protocol and the anti-Caveolin 1 antibody. The second step reagent was FITC goat anti mouse Ig Cat. No. 554001 . Images were taken on a BD Pathway 855 bioimaging system using a 20x objective. This antibody also stained A549 ATCC CCL-185 cells and worked with both the Triton X-100 and alcohol fix/perm protocols see Recommended Assay Procedure . This antibody is not recommended for staining U-2 OS cells. Left: Western blot analysis of Caveolin 1 on a human endothelial lysate. Lane 1: 1:500, lane 2: 1:1000, lane 3: 1:2000 dilution of the Caveolin 1 antibody. Right: Immunofluorescent staining of HeLa ATCC CCL-2 cells. Cells were seeded in a 96 well imaging plate Cat. No. 353219 at 10 000 cells per well. After overnight incubation, cells were stained using the Triton X-100 perm protocol and the anti-Caveolin 1 antibody. The second step reagent was FITC goat anti mouse Ig Cat. No. 554001 . Images were taken on a BD Pathway 855 bioimaging system using a 20x objective. This antibody also stained A549 ATCC CCL-185 cells and worked with both the Triton X-100 and alcohol fix/perm protocols see Recommended Assay Procedure . This antibody is not recommended for staining U-2 OS cells. Show More Left: Western blot analysis of Caveolin 1 on a human endothelial lysate. Lane 1: 1:500, lane 2: 1:1000, lane 3: 1:2000 dilution of the Caveolin 1 antibody. Right: Immunofluorescent staining of HeLa ATCC CCL-2 cells. Cells were seeded in a 96 well imaging plate Cat. No. 353219 at 10 000 cells per well. After overnight incubation, cells were stained using the Triton X-100 perm protocol and the anti-Caveolin 1 antibody. The second step reagent was FITC goat anti mouse Ig Cat. No. 554001 . Images were taken on a BD Pathway 855 bioimaging system using a 20x objective. This antibody also stained A549 ATCC CCL-185 cells and worked with both the Triton X-100 and alcohol fix/perm protocols see Recommended Assay Procedure . This antibody is not recommended for staining U-2 OS cells. Show More Left: Western blot analysis of Caveolin 1 on a human endothelial lysate. Lane 1: 1:500, lane 2: 1:1000, lane 3: 1:2000 dilution of the Caveolin 1 antibody. Right: Immunofluorescent staining of HeLa ATCC CCL-2 cells. Cells were seeded in a 96 well imaging plate Cat. No. 353219 at 10 000 cells per well. After overnight incubation, cells were stained using the Triton X-100 perm protocol and the anti-Caveolin 1 antibody. The second step reagent was FITC goat anti mouse Ig Cat. No. 554001 . Images were taken on a BD Pathway 855 bioimaging system using a 20x objective. This antibody also stained A549 ATCC CCL-185 cells and worked with both the Triton X-100 and alcohol fix/perm protocols see Recommended Assay Procedure . This antibody is not recommended for staining U-2 OS cells. Left: Western blot analysis of Caveolin 1 on a human endothelial lysate. Lane 1: 1:500, lane 2: 1:1000, lane 3: 1:2000 dilution of the Caveolin 1 antibody. Right: Immunofluorescent staining of HeLa ATCC CCL-2 cells. Cells were seeded in a 96 well imaging plate Cat. No. 353219 at 10 000 cells per well. After overnight incubation, cells were stained using the Triton X-100 perm protocol and the anti-Caveolin 1 antibody. The second step reagent was FITC goat anti mouse Ig Cat. No. 554001 . Images were taken on a BD Pathway 855 bioimaging system using a 20x objective. This antibody also stained A549 ATCC CCL-185 cells and worked with both the Triton X-100 and alcohol fix/perm protocols see Recommended Assay Procedure . This antibody is not recommended for staining U-2 OS cells.