BD Transduction LaboratoriesTM Purified Mouse Anti-Crk

Description

Western blot analysis of Crk on a HeLa lysate. Lane 1: 1:5000, lane 2: 1:10000, lane 3: 1:20000 dilution of the Crk antibody. Immunofluorescent staining of HeLa ATCC CCL-2 cells. Cells were seeded in a 96 well imaging plate Cat. No. 353219 at 10 000 cells per well. After overnight incubation, cells were stained using the Triton X-100 perm protocol and the anti-Crk antibody. The second step reagent was FITC goat anti mouse Ig Cat. No. 554001 . Images were taken on a BD Pathway 855 Bioimager system using a 20x objective. This antibody also stained A549 ATCC CCL-185 and U-2 OS ATCC HTB-96 cells and worked with both the Triton X/100 and alcohol perm protocols see Recommended Assay Procedure . Western blot analysis of Crk on a HeLa lysate. Lane 1: 1:5000, lane 2: 1:10000, lane 3: 1:20000 dilution of the Crk antibody. Show More Immunofluorescent staining of HeLa ATCC CCL-2 cells. Cells were seeded in a 96 well imaging plate Cat. No. 353219 at 10 000 cells per well. After overnight incubation, cells were stained using the Triton X-100 perm protocol and the anti-Crk antibody. The second step reagent was FITC goat anti mouse Ig Cat. No. 554001 . Images were taken on a BD Pathway 855 Bioimager system using a 20x objective. This antibody also stained A549 ATCC CCL-185 and U-2 OS ATCC HTB-96 cells and worked with both the Triton X/100 and alcohol perm protocols see Recommended Assay Procedure . Show More Western blot analysis of Crk on a HeLa lysate. Lane 1: 1:5000, lane 2: 1:10000, lane 3: 1:20000 dilution of the Crk antibody. Immunofluorescent staining of HeLa ATCC CCL-2 cells. Cells were seeded in a 96 well imaging plate Cat. No. 353219 at 10 000 cells per well. After overnight incubation, cells were stained using the Triton X-100 perm protocol and the anti-Crk antibody. The second step reagent was FITC goat anti mouse Ig Cat. No. 554001 . Images were taken on a BD Pathway 855 Bioimager system using a 20x objective. This antibody also stained A549 ATCC CCL-185 and U-2 OS ATCC HTB-96 cells and worked with both the Triton X/100 and alcohol perm protocols see Recommended Assay Procedure .