BD Transduction LaboratoriesTM Purified Mouse Anti-E-Cadherin

Description

RIGHT IMAGE: Immunofluorescent staining of WIDR cells at 1:50 dilution using Purified Mouse Anti-E-Cadherin. LEFT IMAGE: Western blot analysis of E-Cadherin using Purified Mouse Anti-E-Cadherin Cat. No. 610181/610182 . E-Cadherin is observable at 120kDa. Left Panel: A431 lysate ATCC CRL-1555; Human epithelial carcinoma was blotted at 1:10000 & 1:20000 Lanes 1 & 2 respectively; 30 second exposure . Middle Left Panel: 293F control lysate was blotted at 1:250 & 1:500 Lanes 1 & 2 respectively; 30 second exposure . Middle Right Panel: 293F cells transfected with human E-Cadherin CDH1 was blotted at 1:2500 & 1: 5000 Lanes 1 & 2 respectively; 5 second exposure . Right Panel: 293 cells transfected with human P-Cadherin CDH3 was blotted using Purified Mouse Anti-E-Cadherin Cat. No. 610181/610182 at 1:2500 & 1: 5000 Lanes 1 & 2 respectively; 5 second exposure . RIGHT IMAGE: Immunofluorescent staining of WIDR cells at 1:50 dilution using Purified Mouse Anti-E-Cadherin. LEFT IMAGE: Western blot analysis of E-Cadherin using Purified Mouse Anti-E-Cadherin Cat. No. 610181/610182 . E-Cadherin is observable at 120kDa. Left Panel: A431 lysate ATCC CRL-1555; Human epithelial carcinoma was blotted at 1:10000 & 1:20000 Lanes 1 & 2 respectively; 30 second exposure . Middle Left Panel: 293F control lysate was blotted at 1:250 & 1:500 Lanes 1 & 2 respectively; 30 second exposure . Middle Right Panel: 293F cells transfected with human E-Cadherin CDH1 was blotted at 1:2500 & 1: 5000 Lanes 1 & 2 respectively; 5 second exposure . Right Panel: 293 cells transfected with human P-Cadherin CDH3 was blotted using Purified Mouse Anti-E-Cadherin Cat. No. 610181/610182 at 1:2500 & 1: 5000 Lanes 1 & 2 respectively; 5 second exposure . RIGHT IMAGE: Immunofluorescent staining of WIDR cells at 1:50 dilution using Purified Mouse Anti-E-Cadherin. LEFT IMAGE: Western blot analysis of E-Cadherin using Purified Mouse Anti-E-Cadherin Cat. No. 610181/610182 . E-Cadherin is observable at 120kDa. Left Panel: A431 lysate ATCC CRL-1555; Human epithelial carcinoma was blotted at 1:10000 & 1:20000 Lanes 1 & 2 respectively; 30 second exposure . Middle Left Panel: 293F control lysate was blotted at 1:250 & 1:500 Lanes 1 & 2 respectively; 30 second exposure . Middle Right Panel: 293F cells transfected with human E-Cadherin CDH1 was blotted at 1:2500 & 1: 5000 Lanes 1 & 2 respectively; 5 second exposure . Right Panel: 293 cells transfected with human P-Cadherin CDH3 was blotted using Purified Mouse Anti-E-Cadherin Cat. No. 610181/610182 at 1:2500 & 1: 5000 Lanes 1 & 2 respectively; 5 second exposure . Show More RIGHT IMAGE: Immunofluorescent staining of WIDR cells at 1:50 dilution using Purified Mouse Anti-E-Cadherin. LEFT IMAGE: Western blot analysis of E-Cadherin using Purified Mouse Anti-E-Cadherin Cat. No. 610181/610182 . E-Cadherin is observable at 120kDa. Left Panel: A431 lysate ATCC CRL-1555; Human epithelial carcinoma was blotted at 1:10000 & 1:20000 Lanes 1 & 2 respectively; 30 second exposure . Middle Left Panel: 293F control lysate was blotted at 1:250 & 1:500 Lanes 1 & 2 respectively; 30 second exposure . Middle Right Panel: 293F cells transfected with human E-Cadherin CDH1 was blotted at 1:2500 & 1: 5000 Lanes 1 & 2 respectively; 5 second exposure . Right Panel: 293 cells transfected with human P-Cadherin CDH3 was blotted using Purified Mouse Anti-E-Cadherin Cat. No. 610181/610182 at 1:2500 & 1: 5000 Lanes 1 & 2 respectively; 5 second exposure . Show More RIGHT IMAGE: Immunofluorescent staining of WIDR cells at 1:50 dilution using Purified Mouse Anti-E-Cadherin. LEFT IMAGE: Western blot analysis of E-Cadherin using Purified Mouse Anti-E-Cadherin Cat. No. 610181/610182 . E-Cadherin is observable at 120kDa. Left Panel: A431 lysate ATCC CRL-1555; Human epithelial carcinoma was blotted at 1:10000 & 1:20000 Lanes 1 & 2 respectively; 30 second exposure . Middle Left Panel: 293F control lysate was blotted at 1:250 & 1:500 Lanes 1 & 2 respectively; 30 second exposure . Middle Right Panel: 293F cells transfected with human E-Cadherin CDH1 was blotted at 1:2500 & 1: 5000 Lanes 1 & 2 respectively; 5 second exposure . Right Panel: 293 cells transfected with human P-Cadherin CDH3 was blotted using Purified Mouse Anti-E-Cadherin Cat. No. 610181/610182 at 1:2500 & 1: 5000 Lanes 1 & 2 respectively; 5 second exposure . RIGHT IMAGE: Immunofluorescent staining of WIDR cells at 1:50 dilution using Purified Mouse Anti-E-Cadherin. LEFT IMAGE: Western blot analysis of E-Cadherin using Purified Mouse Anti-E-Cadherin Cat. No. 610181/610182 . E-Cadherin is observable at 120kDa. Left Panel: A431 lysate ATCC CRL-1555; Human epithelial carcinoma was blotted at 1:10000 & 1:20000 Lanes 1 & 2 respectively; 30 second exposure . Middle Left Panel: 293F control lysate was blotted at 1:250 & 1:500 Lanes 1 & 2 respectively; 30 second exposure . Middle Right Panel: 293F cells transfected with human E-Cadherin CDH1 was blotted at 1:2500 & 1: 5000 Lanes 1 & 2 respectively; 5 second exposure . Right Panel: 293 cells transfected with human P-Cadherin CDH3 was blotted using Purified Mouse Anti-E-Cadherin Cat. No. 610181/610182 at 1:2500 & 1: 5000 Lanes 1 & 2 respectively; 5 second exposure .