BD Transduction LaboratoriesTM Purified Mouse Anti-Fibronectin

Description

Western blot analysis of Fibronectin. A-431 Cell Lysate Cat. No. 611447 was blotted with Purified Mouse Anti-Fibronectin monoclonal antibody at dilutions of 1:5000 Lane 1 , 1:10,000 Lane 2 , and 1:20,000 Lane 3 . Flow cytometric analysis of Fibronectin in human mesenchymal stem cells MSC . MSC Lonza , passage 6, were dissociated and fixed in BD Cytofix Fixation Buffer Cat. No. 554655 and permeabilized with BD Phosflow Perm Buffer III Cat. No. 558050 . Cells were stained with Purified Mouse IgG1, kappa isotype control dashed line, Cat. No. 349040 or Purified Mouse Anti-Fibronectin monoclonal antibody solid line, Cat. No. 610077 at matched concentrations. The secondary antibody was FITC Goat Anti-Mouse Ig Cat No. 554001 . Histograms were derived from gated events based on light scattering characteristics of MSCs. Flow cytometry was performed on a BD LSRFortessa II flow cytometry system. BD Phosflow Perm/Wash Buffer I Cat. No. 557885 is also suitable for permeabilization. Immunofluorescent analysis of Fibronectin in human mesenchymal stem cells MSC . MSC Lonza , passage 6, were fixed in BD Cytofix Fixation Buffer Cat. No. 554655 , permeabilized with 0.1 % Triton X-100 and stained with Purified Mouse Anti-Fibronectin monoclonal antibody Cat. No.610077, pseudo-colored green at 2.5 mug/ml. The second-step reagent was Alexa Fluor 488 goat anti-mouse Ig Life Technologies , and counter-staining of cell nuclei was with DAPI pseudo-colored blue . The images were captured on a BD Pathway 435 Cell Analyzer and merged using BD AttovisionSoftware. Western blot analysis of Fibronectin. A-431 Cell Lysate Cat. No. 611447 was blotted with Purified Mouse Anti-Fibronectin monoclonal antibody at dilutions of 1:5000 Lane 1 , 1:10,000 Lane 2 , and 1:20,000 Lane 3 . Show More Flow cytometric analysis of Fibronectin in human mesenchymal stem cells MSC . MSC Lonza , passage 6, were dissociated and fixed in BD Cytofix Fixation Buffer Cat. No. 554655 and permeabilized with BD Phosflow Perm Buffer III Cat. No. 558050 . Cells were stained with Purified Mouse IgG1, kappa isotype control dashed line, Cat. No. 349040 or Purified Mouse Anti-Fibronectin monoclonal antibody solid line, Cat. No. 610077 at matched concentrations. The secondary antibody was FITC Goat Anti-Mouse Ig Cat No. 554001 . Histograms were derived from gated events based on light scattering characteristics of MSCs. Flow cytometry was performed on a BD LSRFortessa II flow cytometry system. BD Phosflow Perm/Wash Buffer I Cat. No. 557885 is also suitable for permeabilization. Show More Immunofluorescent analysis of Fibronectin in human mesenchymal stem cells MSC . MSC Lonza , passage 6, were fixed in BD Cytofix Fixation Buffer Cat. No. 554655 , permeabilized with 0.1 % Triton X-100 and stained with Purified Mouse Anti-Fibronectin monoclonal antibody Cat. No.610077, pseudo-colored green at 2.5 mug/ml. The second-step reagent was Alexa Fluor 488 goat anti-mouse Ig Life Technologies , and counter-staining of cell nuclei was with DAPI pseudo-colored blue . The images were captured on a BD Pathway 435 Cell Analyzer and merged using BD AttovisionSoftware. Show More Western blot analysis of Fibronectin. A-431 Cell Lysate Cat. No. 611447 was blotted with Purified Mouse Anti-Fibronectin monoclonal antibody at dilutions of 1:5000 Lane 1 , 1:10,000 Lane 2 , and 1:20,000 Lane 3 . Flow cytometric analysis of Fibronectin in human mesenchymal stem cells MSC . MSC Lonza , passage 6, were dissociated and fixed in BD Cytofix Fixation Buffer Cat. No. 554655 and permeabilized with BD Phosflow Perm Buffer III Cat. No. 558050 . Cells were stained with Purified Mouse IgG1, kappa isotype control dashed line, Cat. No. 349040 or Purified Mouse Anti-Fibronectin monoclonal antibody solid line, Cat. No. 610077 at matched concentrations. The secondary antibody was FITC Goat Anti-Mouse Ig Cat No. 554001 . Histograms were derived from gated events based on light scattering characteristics of MSCs. Flow cytometry was performed on a BD LSRFortessa II flow cytometry system. BD Phosflow Perm/Wash Buffer I Cat. No. 557885 is also suitable for permeabilization. Immunofluorescent analysis of Fibronectin in human mesenchymal stem cells MSC . MSC Lonza , passage 6, were fixed in BD Cytofix Fixation Buffer Cat. No. 554655 , permeabilized with 0.1 % Triton X-100 and stained with Purified Mouse Anti-Fibronectin monoclonal antibody Cat. No.610077, pseudo-colored green at 2.5 mug/ml. The second-step reagent was Alexa Fluor 488 goat anti-mouse Ig Life Technologies , and counter-staining of cell nuclei was with DAPI pseudo-colored blue . The images were captured on a BD Pathway 435 Cell Analyzer and merged using BD AttovisionSoftware.