BD Transduction LaboratoriesTM Purified Mouse Anti-p38 MAPK (pT180/pY182)

Description

Western blot analysis for p38 MAPK pT180/pY182 left figure . HeLa cells Human cervical epitheloid carcinoma; ATCC CCL-2 were either left untreated lane 1 or treated with 25 mug/mL anisomycin, an antibiotic and protein synthesis inhibitor, for 15 min at 37C lane 2 . The top panel was probed with a mouse anti-p38alpha antibody cat.# 612168 and the bottom was probed with the mouse anti-p38 pT180/pY182 antibody. Immunofluorescence staining for p38 MAPK pT180/pY182 right figure . HeLa cells Human cervical epitheloid carcinoma; ATCC CCL-2 were either untreated upper left and bottom left panels or were treated for 15 min with 25 mug/mL anisomycin, an antibiotic and protein synthesis inhibitor upper right and bottom right panels . Cells were fixed in 3.75 % paraformaldehyde and permeabilized with 0.2 % Triton-X 100. Immunofluorescent staining was performed with either a mouse anti-p38alpha antibody cat.#612168 upper left and upper right panels or with the mouse anti-p38 MAPK pT180/pY182 antibody bottom left and bottom right panels . Western blot analysis for p38 MAPK pT180/pY182 left figure . HeLa cells Human cervical epitheloid carcinoma; ATCC CCL-2 were either left untreated lane 1 or treated with 25 mug/mL anisomycin, an antibiotic and protein synthesis inhibitor, for 15 min at 37C lane 2 . The top panel was probed with a mouse anti-p38alpha antibody cat.# 612168 and the bottom was probed with the mouse anti-p38 pT180/pY182 antibody. Immunofluorescence staining for p38 MAPK pT180/pY182 right figure . HeLa cells Human cervical epitheloid carcinoma; ATCC CCL-2 were either untreated upper left and bottom left panels or were treated for 15 min with 25 mug/mL anisomycin, an antibiotic and protein synthesis inhibitor upper right and bottom right panels . Cells were fixed in 3.75 % paraformaldehyde and permeabilized with 0.2 % Triton-X 100. Immunofluorescent staining was performed with either a mouse anti-p38alpha antibody cat.#612168 upper left and upper right panels or with the mouse anti-p38 MAPK pT180/pY182 antibody bottom left and bottom right panels . Western blot analysis for p38 MAPK pT180/pY182 left figure . HeLa cells Human cervical epitheloid carcinoma; ATCC CCL-2 were either left untreated lane 1 or treated with 25 mug/mL anisomycin, an antibiotic and protein synthesis inhibitor, for 15 min at 37C lane 2 . The top panel was probed with a mouse anti-p38alpha antibody cat.# 612168 and the bottom was probed with the mouse anti-p38 pT180/pY182 antibody. Immunofluorescence staining for p38 MAPK pT180/pY182 right figure . HeLa cells Human cervical epitheloid carcinoma; ATCC CCL-2 were either untreated upper left and bottom left panels or were treated for 15 min with 25 mug/mL anisomycin, an antibiotic and protein synthesis inhibitor upper right and bottom right panels . Cells were fixed in 3.75 % paraformaldehyde and permeabilized with 0.2 % Triton-X 100. Immunofluorescent staining was performed with either a mouse anti-p38alpha antibody cat.#612168 upper left and upper right panels or with the mouse anti-p38 MAPK pT180/pY182 antibody bottom left and bottom right panels . Show More Western blot analysis for p38 MAPK pT180/pY182 left figure . HeLa cells Human cervical epitheloid carcinoma; ATCC CCL-2 were either left untreated lane 1 or treated with 25 mug/mL anisomycin, an antibiotic and protein synthesis inhibitor, for 15 min at 37C lane 2 . The top panel was probed with a mouse anti-p38alpha antibody cat.# 612168 and the bottom was probed with the mouse anti-p38 pT180/pY182 antibody. Immunofluorescence staining for p38 MAPK pT180/pY182 right figure . HeLa cells Human cervical epitheloid carcinoma; ATCC CCL-2 were either untreated upper left and bottom left panels or were treated for 15 min with 25 mug/mL anisomycin, an antibiotic and protein synthesis inhibitor upper right and bottom right panels . Cells were fixed in 3.75 % paraformaldehyde and permeabilized with 0.2 % Triton-X 100. Immunofluorescent staining was performed with either a mouse anti-p38alpha antibody cat.#612168 upper left and upper right panels or with the mouse anti-p38 MAPK pT180/pY182 antibody bottom left and bottom right panels . Show More Western blot analysis for p38 MAPK pT180/pY182 left figure . HeLa cells Human cervical epitheloid carcinoma; ATCC CCL-2 were either left untreated lane 1 or treated with 25 mug/mL anisomycin, an antibiotic and protein synthesis inhibitor, for 15 min at 37C lane 2 . The top panel was probed with a mouse anti-p38alpha antibody cat.# 612168 and the bottom was probed with the mouse anti-p38 pT180/pY182 antibody. Immunofluorescence staining for p38 MAPK pT180/pY182 right figure . HeLa cells Human cervical epitheloid carcinoma; ATCC CCL-2 were either untreated upper left and bottom left panels or were treated for 15 min with 25 mug/mL anisomycin, an antibiotic and protein synthesis inhibitor upper right and bottom right panels . Cells were fixed in 3.75 % paraformaldehyde and permeabilized with 0.2 % Triton-X 100. Immunofluorescent staining was performed with either a mouse anti-p38alpha antibody cat.#612168 upper left and upper right panels or with the mouse anti-p38 MAPK pT180/pY182 antibody bottom left and bottom right panels . Western blot analysis for p38 MAPK pT180/pY182 left figure . HeLa cells Human cervical epitheloid carcinoma; ATCC CCL-2 were either left untreated lane 1 or treated with 25 mug/mL anisomycin, an antibiotic and protein synthesis inhibitor, for 15 min at 37C lane 2 . The top panel was probed with a mouse anti-p38alpha antibody cat.# 612168 and the bottom was probed with the mouse anti-p38 pT180/pY182 antibody. Immunofluorescence staining for p38 MAPK pT180/pY182 right figure . HeLa cells Human cervical epitheloid carcinoma; ATCC CCL-2 were either untreated upper left and bottom left panels or were treated for 15 min with 25 mug/mL anisomycin, an antibiotic and protein synthesis inhibitor upper right and bottom right panels . Cells were fixed in 3.75 % paraformaldehyde and permeabilized with 0.2 % Triton-X 100. Immunofluorescent staining was performed with either a mouse anti-p38alpha antibody cat.#612168 upper left and upper right panels or with the mouse anti-p38 MAPK pT180/pY182 antibody bottom left and bottom right panels .