BD Transduction LaboratoriesTM Purified Mouse Anti-Phospholipase Cgamma1

Description

Western blot analysis of Phospholipase Cgamma1 on a A431 cell lysate Human epithelial carcinoma; ATCC CRL-1555 . Lane 1: 1:250, lane 2: 1:500, lane 3: 1:1000 dilution of the mouse anti-Phospholipase Cgamma1 antibody. Western blot analysis of Phospholipase Cgamma1 on a A431 cell lysate Human epithelial carcinoma; ATCC CRL-1555 . Lane 1: 1:250, lane 2: 1:500, lane 3: 1:1000 dilution of the mouse anti-Phospholipase Cgamma1 antibody. Immunofluorescence staining of HeLa cells. Cells were seeded in a 96 well imaging plate Cat. No. 353219 at 10,000 cells per well. After overnight incubation, cells were stained using the methanol fix/perm protocol see Recommended Assay Procedure and the purified mouse anti-Phospholipase Cgamma1 antibody. The second step reagent was FITC goat anti-mouse Ig Cat. No. 554001 . The image was taken on a BD Pathway 850 imager using a 20x objective. This antibody also stained A549 and U2OS cells and can be used with either fix/perm protocol see Recommended Assay Procedure . Western blot analysis of Phospholipase Cgamma1 on a A431 cell lysate Human epithelial carcinoma; ATCC CRL-1555 . Lane 1: 1:250, lane 2: 1:500, lane 3: 1:1000 dilution of the mouse anti-Phospholipase Cgamma1 antibody. Show More Western blot analysis of Phospholipase Cgamma1 on a A431 cell lysate Human epithelial carcinoma; ATCC CRL-1555 . Lane 1: 1:250, lane 2: 1:500, lane 3: 1:1000 dilution of the mouse anti-Phospholipase Cgamma1 antibody. Show More Immunofluorescence staining of HeLa cells. Cells were seeded in a 96 well imaging plate Cat. No. 353219 at 10,000 cells per well. After overnight incubation, cells were stained using the methanol fix/perm protocol see Recommended Assay Procedure and the purified mouse anti-Phospholipase Cgamma1 antibody. The second step reagent was FITC goat anti-mouse Ig Cat. No. 554001 . The image was taken on a BD Pathway 850 imager using a 20x objective. This antibody also stained A549 and U2OS cells and can be used with either fix/perm protocol see Recommended Assay Procedure . Show More Western blot analysis of Phospholipase Cgamma1 on a A431 cell lysate Human epithelial carcinoma; ATCC CRL-1555 . Lane 1: 1:250, lane 2: 1:500, lane 3: 1:1000 dilution of the mouse anti-Phospholipase Cgamma1 antibody. Western blot analysis of Phospholipase Cgamma1 on a A431 cell lysate Human epithelial carcinoma; ATCC CRL-1555 . Lane 1: 1:250, lane 2: 1:500, lane 3: 1:1000 dilution of the mouse anti-Phospholipase Cgamma1 antibody. Immunofluorescence staining of HeLa cells. Cells were seeded in a 96 well imaging plate Cat. No. 353219 at 10,000 cells per well. After overnight incubation, cells were stained using the methanol fix/perm protocol see Recommended Assay Procedure and the purified mouse anti-Phospholipase Cgamma1 antibody. The second step reagent was FITC goat anti-mouse Ig Cat. No. 554001 . The image was taken on a BD Pathway 850 imager using a 20x objective. This antibody also stained A549 and U2OS cells and can be used with either fix/perm protocol see Recommended Assay Procedure .