BD Transduction LaboratoriesTM Purified Mouse Anti-Phospholipase Cbeta4

Description

Western blot analysis of Phospholipase Cbeta4 expression on a rat pituitary lysate left panel . Rat lysate was stained with Purified Mouse Anti-Phospholipase Cbeta4 Cat. No. 611540 at dilutions of 1: 1:250, 1:500, and 1:1000 lanes 1, 2, and 3 respectively . Purified Mouse Anti-Phospholipase Cbeta4 expression was visualized with HRP Goat Anti-Mouse Ig Cat. No. 554002 and appears as a 130 kDa band. Immunofluorescent analysis of Phospholipase Cbeta4 expression on C6 cells Rat glioma; ATCC CCL-107 right . Cells were seeded in a 384-well collagen coated microplate at 6,000 cells per well. After overnight incubation, cells were stained using the Triton-X 100 fix/perm protocol see Recommended Assay Procedure and Purified Mouse Anti-Phospholipase Cbeta4. The second step reagent was Alexa Fluor 488 goat anti-mouse Ig Invitrogen . The image was taken on a BD Pathway 855 or 435 Bioimager using a 20x objective. This antibody also stained SH-SY5Y Human neuroblastoma; ATCC CRL-2266 and SK-N-SH cells Human neuroblastoma; ATCC HTB-11 using both the Triton-X 100 and methanol fix/perm protocols see Recommended Assay Procedure . Western blot analysis of Phospholipase Cbeta4 expression on a rat pituitary lysate left panel . Rat lysate was stained with Purified Mouse Anti-Phospholipase Cbeta4 Cat. No. 611540 at dilutions of 1: 1:250, 1:500, and 1:1000 lanes 1, 2, and 3 respectively . Purified Mouse Anti-Phospholipase Cbeta4 expression was visualized with HRP Goat Anti-Mouse Ig Cat. No. 554002 and appears as a 130 kDa band. Immunofluorescent analysis of Phospholipase Cbeta4 expression on C6 cells Rat glioma; ATCC CCL-107 right . Cells were seeded in a 384-well collagen coated microplate at 6,000 cells per well. After overnight incubation, cells were stained using the Triton-X 100 fix/perm protocol see Recommended Assay Procedure and Purified Mouse Anti-Phospholipase Cbeta4. The second step reagent was Alexa Fluor 488 goat anti-mouse Ig Invitrogen . The image was taken on a BD Pathway 855 or 435 Bioimager using a 20x objective. This antibody also stained SH-SY5Y Human neuroblastoma; ATCC CRL-2266 and SK-N-SH cells Human neuroblastoma; ATCC HTB-11 using both the Triton-X 100 and methanol fix/perm protocols see Recommended Assay Procedure . Western blot analysis of Phospholipase Cbeta4 expression on a rat pituitary lysate left panel . Rat lysate was stained with Purified Mouse Anti-Phospholipase Cbeta4 Cat. No. 611540 at dilutions of 1: 1:250, 1:500, and 1:1000 lanes 1, 2, and 3 respectively . Purified Mouse Anti-Phospholipase Cbeta4 expression was visualized with HRP Goat Anti-Mouse Ig Cat. No. 554002 and appears as a 130 kDa band. Immunofluorescent analysis of Phospholipase Cbeta4 expression on C6 cells Rat glioma; ATCC CCL-107 right . Cells were seeded in a 384-well collagen coated microplate at 6,000 cells per well. After overnight incubation, cells were stained using the Triton-X 100 fix/perm protocol see Recommended Assay Procedure and Purified Mouse Anti-Phospholipase Cbeta4. The second step reagent was Alexa Fluor 488 goat anti-mouse Ig Invitrogen . The image was taken on a BD Pathway 855 or 435 Bioimager using a 20x objective. This antibody also stained SH-SY5Y Human neuroblastoma; ATCC CRL-2266 and SK-N-SH cells Human neuroblastoma; ATCC HTB-11 using both the Triton-X 100 and methanol fix/perm protocols see Recommended Assay Procedure . Show More Western blot analysis of Phospholipase Cbeta4 expression on a rat pituitary lysate left panel . Rat lysate was stained with Purified Mouse Anti-Phospholipase Cbeta4 Cat. No. 611540 at dilutions of 1: 1:250, 1:500, and 1:1000 lanes 1, 2, and 3 respectively . Purified Mouse Anti-Phospholipase Cbeta4 expression was visualized with HRP Goat Anti-Mouse Ig Cat. No. 554002 and appears as a 130 kDa band. Immunofluorescent analysis of Phospholipase Cbeta4 expression on C6 cells Rat glioma; ATCC CCL-107 right . Cells were seeded in a 384-well collagen coated microplate at 6,000 cells per well. After overnight incubation, cells were stained using the Triton-X 100 fix/perm protocol see Recommended Assay Procedure and Purified Mouse Anti-Phospholipase Cbeta4. The second step reagent was Alexa Fluor 488 goat anti-mouse Ig Invitrogen . The image was taken on a BD Pathway 855 or 435 Bioimager using a 20x objective. This antibody also stained SH-SY5Y Human neuroblastoma; ATCC CRL-2266 and SK-N-SH cells Human neuroblastoma; ATCC HTB-11 using both the Triton-X 100 and methanol fix/perm protocols see Recommended Assay Procedure . Show More Western blot analysis of Phospholipase Cbeta4 expression on a rat pituitary lysate left panel . Rat lysate was stained with Purified Mouse Anti-Phospholipase Cbeta4 Cat. No. 611540 at dilutions of 1: 1:250, 1:500, and 1:1000 lanes 1, 2, and 3 respectively . Purified Mouse Anti-Phospholipase Cbeta4 expression was visualized with HRP Goat Anti-Mouse Ig Cat. No. 554002 and appears as a 130 kDa band. Immunofluorescent analysis of Phospholipase Cbeta4 expression on C6 cells Rat glioma; ATCC CCL-107 right . Cells were seeded in a 384-well collagen coated microplate at 6,000 cells per well. After overnight incubation, cells were stained using the Triton-X 100 fix/perm protocol see Recommended Assay Procedure and Purified Mouse Anti-Phospholipase Cbeta4. The second step reagent was Alexa Fluor 488 goat anti-mouse Ig Invitrogen . The image was taken on a BD Pathway 855 or 435 Bioimager using a 20x objective. This antibody also stained SH-SY5Y Human neuroblastoma; ATCC CRL-2266 and SK-N-SH cells Human neuroblastoma; ATCC HTB-11 using both the Triton-X 100 and methanol fix/perm protocols see Recommended Assay Procedure . Western blot analysis of Phospholipase Cbeta4 expression on a rat pituitary lysate left panel . Rat lysate was stained with Purified Mouse Anti-Phospholipase Cbeta4 Cat. No. 611540 at dilutions of 1: 1:250, 1:500, and 1:1000 lanes 1, 2, and 3 respectively . Purified Mouse Anti-Phospholipase Cbeta4 expression was visualized with HRP Goat Anti-Mouse Ig Cat. No. 554002 and appears as a 130 kDa band. Immunofluorescent analysis of Phospholipase Cbeta4 expression on C6 cells Rat glioma; ATCC CCL-107 right . Cells were seeded in a 384-well collagen coated microplate at 6,000 cells per well. After overnight incubation, cells were stained using the Triton-X 100 fix/perm protocol see Recommended Assay Procedure and Purified Mouse Anti-Phospholipase Cbeta4. The second step reagent was Alexa Fluor 488 goat anti-mouse Ig Invitrogen . The image was taken on a BD Pathway 855 or 435 Bioimager using a 20x objective. This antibody also stained SH-SY5Y Human neuroblastoma; ATCC CRL-2266 and SK-N-SH cells Human neuroblastoma; ATCC HTB-11 using both the Triton-X 100 and methanol fix/perm protocols see Recommended Assay Procedure .