BD Transduction LaboratoriesTM Purified Mouse Anti-RACK1

Description

Western blot analysis of RACK1 on a Jurkat lysate. Lane 1: 1:2500, lane 2: 1:5000, lane 3: 1:10000 dilution of the RACK1 antibody. Immunofluorescent staining of A549 ATCC CCL-185 cells. Cells were seeded in a 96 well imaging plate Cat. No. 353219 at 10 000 cells per well. After overnight incubation, cells were stained using the alcohol perm protocol and the anti-RACK1 antibody. The second step reagent was FITC goat anti mouse Ig Cat. No. 554001 . The image was taken on a BD Pathway 855 Bioimager using a 20x objective. This antibody also stained U-2 OS ATCC HTB-96 and HeLa ATCC CCL-2 cells using both the Triton X-100 and alcohol perm protocols see Recommended Assay Procedure . Western blot analysis of RACK1 on a Jurkat lysate. Lane 1: 1:2500, lane 2: 1:5000, lane 3: 1:10000 dilution of the RACK1 antibody. Show More Immunofluorescent staining of A549 ATCC CCL-185 cells. Cells were seeded in a 96 well imaging plate Cat. No. 353219 at 10 000 cells per well. After overnight incubation, cells were stained using the alcohol perm protocol and the anti-RACK1 antibody. The second step reagent was FITC goat anti mouse Ig Cat. No. 554001 . The image was taken on a BD Pathway 855 Bioimager using a 20x objective. This antibody also stained U-2 OS ATCC HTB-96 and HeLa ATCC CCL-2 cells using both the Triton X-100 and alcohol perm protocols see Recommended Assay Procedure . Show More Western blot analysis of RACK1 on a Jurkat lysate. Lane 1: 1:2500, lane 2: 1:5000, lane 3: 1:10000 dilution of the RACK1 antibody. Immunofluorescent staining of A549 ATCC CCL-185 cells. Cells were seeded in a 96 well imaging plate Cat. No. 353219 at 10 000 cells per well. After overnight incubation, cells were stained using the alcohol perm protocol and the anti-RACK1 antibody. The second step reagent was FITC goat anti mouse Ig Cat. No. 554001 . The image was taken on a BD Pathway 855 Bioimager using a 20x objective. This antibody also stained U-2 OS ATCC HTB-96 and HeLa ATCC CCL-2 cells using both the Triton X-100 and alcohol perm protocols see Recommended Assay Procedure .