BD Transduction LaboratoriesTM Purified Mouse Anti-S100B

Description

Western blot analysis of S100B on a mouse cerebellum lysate left . Lane 1: 1:5000, lane 2: 1:10,000, lane 3: 1:20,000 dilution of the mouse anti-S100B antibody. Immunofluorescent staining of differentiated SH-SY5Y cells Human neuroblastoma; ATCC CRL-2266 right . Cells were seeded in collagen coated 96-well imaging microplates Material # 353219 at 5,000 cells per well. Cells were incubated with 50 muM all-trans-retinoic acid ATRA Sigma-Aldrich, cat.no. R2625 for 5 days, followed by 50 ng/ml BDNF Sigma-Aldrich, cat.no. B3795 for 5 days. Differentiated cells were fixed and stained using the methanol fix/perm protocol see Recommended Assay Procedure and the mouse anti-S100B antibody. The second step reagent was Alexa Fluor 488 goat anti-mouse Ig Invitrogen . The image was taken on a BD Pathway 855 or 435 Bioimager system using a 20x objective. This antibody also stained undifferentiated SK-N-SH Human neuroblastoma; ATCC HTB-11 , C6 Rat glioma; ATCC CCL-107 , U-87 MG Human glioblastoma cells; ATCC HTB-14 and U-373 cells Human glioblastoma cells; ATCC HTB-17; discontinued, investigators may refer to: http://www.atcc.org/MisidentifiedCellLines/tabid/683/Default.aspx using both the Triton-X 100 and methanol fix/perm protocols see Recommended Assay Procedure . Western blot analysis of S100B on a mouse cerebellum lysate left . Lane 1: 1:5000, lane 2: 1:10,000, lane 3: 1:20,000 dilution of the mouse anti-S100B antibody. Immunofluorescent staining of differentiated SH-SY5Y cells Human neuroblastoma; ATCC CRL-2266 right . Cells were seeded in collagen coated 96-well imaging microplates Material # 353219 at 5,000 cells per well. Cells were incubated with 50 muM all-trans-retinoic acid ATRA Sigma-Aldrich, cat.no. R2625 for 5 days, followed by 50 ng/ml BDNF Sigma-Aldrich, cat.no. B3795 for 5 days. Differentiated cells were fixed and stained using the methanol fix/perm protocol see Recommended Assay Procedure and the mouse anti-S100B antibody. The second step reagent was Alexa Fluor 488 goat anti-mouse Ig Invitrogen . The image was taken on a BD Pathway 855 or 435 Bioimager system using a 20x objective. This antibody also stained undifferentiated SK-N-SH Human neuroblastoma; ATCC HTB-11 , C6 Rat glioma; ATCC CCL-107 , U-87 MG Human glioblastoma cells; ATCC HTB-14 and U-373 cells Human glioblastoma cells; ATCC HTB-17; discontinued, investigators may refer to: http://www.atcc.org/MisidentifiedCellLines/tabid/683/Default.aspx using both the Triton-X 100 and methanol fix/perm protocols see Recommended Assay Procedure . Western blot analysis of S100B on a mouse cerebellum lysate left . Lane 1: 1:5000, lane 2: 1:10,000, lane 3: 1:20,000 dilution of the mouse anti-S100B antibody. Immunofluorescent staining of differentiated SH-SY5Y cells Human neuroblastoma; ATCC CRL-2266 right . Cells were seeded in collagen coated 96-well imaging microplates Material # 353219 at 5,000 cells per well. Cells were incubated with 50 muM all-trans-retinoic acid ATRA Sigma-Aldrich, cat.no. R2625 for 5 days, followed by 50 ng/ml BDNF Sigma-Aldrich, cat.no. B3795 for 5 days. Differentiated cells were fixed and stained using the methanol fix/perm protocol see Recommended Assay Procedure and the mouse anti-S100B antibody. The second step reagent was Alexa Fluor 488 goat anti-mouse Ig Invitrogen . The image was taken on a BD Pathway 855 or 435 Bioimager system using a 20x objective. This antibody also stained undifferentiated SK-N-SH Human neuroblastoma; ATCC HTB-11 , C6 Rat glioma; ATCC CCL-107 , U-87 MG Human glioblastoma cells; ATCC HTB-14 and U-373 cells Human glioblastoma cells; ATCC HTB-17; discontinued, investigators may refer to: http://www.atcc.org/MisidentifiedCellLines/tabid/683/Default.aspx using both the Triton-X 100 and methanol fix/perm protocols see Recommended Assay Procedure . Show More Western blot analysis of S100B on a mouse cerebellum lysate left . Lane 1: 1:5000, lane 2: 1:10,000, lane 3: 1:20,000 dilution of the mouse anti-S100B antibody. Immunofluorescent staining of differentiated SH-SY5Y cells Human neuroblastoma; ATCC CRL-2266 right . Cells were seeded in collagen coated 96-well imaging microplates Material # 353219 at 5,000 cells per well. Cells were incubated with 50 muM all-trans-retinoic acid ATRA Sigma-Aldrich, cat.no. R2625 for 5 days, followed by 50 ng/ml BDNF Sigma-Aldrich, cat.no. B3795 for 5 days. Differentiated cells were fixed and stained using the methanol fix/perm protocol see Recommended Assay Procedure and the mouse anti-S100B antibody. The second step reagent was Alexa Fluor 488 goat anti-mouse Ig Invitrogen . The image was taken on a BD Pathway 855 or 435 Bioimager system using a 20x objective. This antibody also stained undifferentiated SK-N-SH Human neuroblastoma; ATCC HTB-11 , C6 Rat glioma; ATCC CCL-107 , U-87 MG Human glioblastoma cells; ATCC HTB-14 and U-373 cells Human glioblastoma cells; ATCC HTB-17; discontinued, investigators may refer to: http://www.atcc.org/MisidentifiedCellLines/tabid/683/Default.aspx using both the Triton-X 100 and methanol fix/perm protocols see Recommended Assay Procedure . Show More Western blot analysis of S100B on a mouse cerebellum lysate left . Lane 1: 1:5000, lane 2: 1:10,000, lane 3: 1:20,000 dilution of the mouse anti-S100B antibody. Immunofluorescent staining of differentiated SH-SY5Y cells Human neuroblastoma; ATCC CRL-2266 right . Cells were seeded in collagen coated 96-well imaging microplates Material # 353219 at 5,000 cells per well. Cells were incubated with 50 muM all-trans-retinoic acid ATRA Sigma-Aldrich, cat.no. R2625 for 5 days, followed by 50 ng/ml BDNF Sigma-Aldrich, cat.no. B3795 for 5 days. Differentiated cells were fixed and stained using the methanol fix/perm protocol see Recommended Assay Procedure and the mouse anti-S100B antibody. The second step reagent was Alexa Fluor 488 goat anti-mouse Ig Invitrogen . The image was taken on a BD Pathway 855 or 435 Bioimager system using a 20x objective. This antibody also stained undifferentiated SK-N-SH Human neuroblastoma; ATCC HTB-11 , C6 Rat glioma; ATCC CCL-107 , U-87 MG Human glioblastoma cells; ATCC HTB-14 and U-373 cells Human glioblastoma cells; ATCC HTB-17; discontinued, investigators may refer to: http://www.atcc.org/MisidentifiedCellLines/tabid/683/Default.aspx using both the Triton-X 100 and methanol fix/perm protocols see Recommended Assay Procedure . Western blot analysis of S100B on a mouse cerebellum lysate left . Lane 1: 1:5000, lane 2: 1:10,000, lane 3: 1:20,000 dilution of the mouse anti-S100B antibody. Immunofluorescent staining of differentiated SH-SY5Y cells Human neuroblastoma; ATCC CRL-2266 right . Cells were seeded in collagen coated 96-well imaging microplates Material # 353219 at 5,000 cells per well. Cells were incubated with 50 muM all-trans-retinoic acid ATRA Sigma-Aldrich, cat.no. R2625 for 5 days, followed by 50 ng/ml BDNF Sigma-Aldrich, cat.no. B3795 for 5 days. Differentiated cells were fixed and stained using the methanol fix/perm protocol see Recommended Assay Procedure and the mouse anti-S100B antibody. The second step reagent was Alexa Fluor 488 goat anti-mouse Ig Invitrogen . The image was taken on a BD Pathway 855 or 435 Bioimager system using a 20x objective. This antibody also stained undifferentiated SK-N-SH Human neuroblastoma; ATCC HTB-11 , C6 Rat glioma; ATCC CCL-107 , U-87 MG Human glioblastoma cells; ATCC HTB-14 and U-373 cells Human glioblastoma cells; ATCC HTB-17; discontinued, investigators may refer to: http://www.atcc.org/MisidentifiedCellLines/tabid/683/Default.aspx using both the Triton-X 100 and methanol fix/perm protocols see Recommended Assay Procedure .