BD Transduction LaboratoriesTM Purified Mouse Anti-Stat1

Description

Left Figure: Western blot analysis of Stat1 on a A431 lysate. Lane 1: 1:1000, lane 2: 1:2000, lane 3: 1:4000 dilution of the anti-Stat1 antibody. Right Figure: Immunofluorescent staining of HeLa ATCC CCL-2 cells. Cells were seeded in a 96 well imaging plate Cat. No. 353219 at 10,000 cells per well. After overnight incubation, cells were either mock treated media, left or exposed to IFN-gamma 100 ng/ml, right for 15 minutes. After treatment, cells were stained using the alcohol perm protocol and the anti-Stat1 antibody. The second step reagent was Alexa-Fluor 555 anti-mouse IgG Invitrogen . The image was taken on a BD Pathway 855 Bioimager using a 20x objective. This antibody also stained A549 ATCC CCL-185 and U-2 OS ATCC HTB-96 cells. The recommended permeabilization agent is Perm Buffer III see recommended assay procedure . Left Figure: Western blot analysis of Stat1 on a A431 lysate. Lane 1: 1:1000, lane 2: 1:2000, lane 3: 1:4000 dilution of the anti-Stat1 antibody. Right Figure: Immunofluorescent staining of HeLa ATCC CCL-2 cells. Cells were seeded in a 96 well imaging plate Cat. No. 353219 at 10,000 cells per well. After overnight incubation, cells were either mock treated media, left or exposed to IFN-gamma 100 ng/ml, right for 15 minutes. After treatment, cells were stained using the alcohol perm protocol and the anti-Stat1 antibody. The second step reagent was Alexa-Fluor 555 anti-mouse IgG Invitrogen . The image was taken on a BD Pathway 855 Bioimager using a 20x objective. This antibody also stained A549 ATCC CCL-185 and U-2 OS ATCC HTB-96 cells. The recommended permeabilization agent is Perm Buffer III see recommended assay procedure . Left Figure: Western blot analysis of Stat1 on a A431 lysate. Lane 1: 1:1000, lane 2: 1:2000, lane 3: 1:4000 dilution of the anti-Stat1 antibody. Right Figure: Immunofluorescent staining of HeLa ATCC CCL-2 cells. Cells were seeded in a 96 well imaging plate Cat. No. 353219 at 10,000 cells per well. After overnight incubation, cells were either mock treated media, left or exposed to IFN-gamma 100 ng/ml, right for 15 minutes. After treatment, cells were stained using the alcohol perm protocol and the anti-Stat1 antibody. The second step reagent was Alexa-Fluor 555 anti-mouse IgG Invitrogen . The image was taken on a BD Pathway 855 Bioimager using a 20x objective. This antibody also stained A549 ATCC CCL-185 and U-2 OS ATCC HTB-96 cells. The recommended permeabilization agent is Perm Buffer III see recommended assay procedure . Show More Left Figure: Western blot analysis of Stat1 on a A431 lysate. Lane 1: 1:1000, lane 2: 1:2000, lane 3: 1:4000 dilution of the anti-Stat1 antibody. Right Figure: Immunofluorescent staining of HeLa ATCC CCL-2 cells. Cells were seeded in a 96 well imaging plate Cat. No. 353219 at 10,000 cells per well. After overnight incubation, cells were either mock treated media, left or exposed to IFN-gamma 100 ng/ml, right for 15 minutes. After treatment, cells were stained using the alcohol perm protocol and the anti-Stat1 antibody. The second step reagent was Alexa-Fluor 555 anti-mouse IgG Invitrogen . The image was taken on a BD Pathway 855 Bioimager using a 20x objective. This antibody also stained A549 ATCC CCL-185 and U-2 OS ATCC HTB-96 cells. The recommended permeabilization agent is Perm Buffer III see recommended assay procedure . Show More Left Figure: Western blot analysis of Stat1 on a A431 lysate. Lane 1: 1:1000, lane 2: 1:2000, lane 3: 1:4000 dilution of the anti-Stat1 antibody. Right Figure: Immunofluorescent staining of HeLa ATCC CCL-2 cells. Cells were seeded in a 96 well imaging plate Cat. No. 353219 at 10,000 cells per well. After overnight incubation, cells were either mock treated media, left or exposed to IFN-gamma 100 ng/ml, right for 15 minutes. After treatment, cells were stained using the alcohol perm protocol and the anti-Stat1 antibody. The second step reagent was Alexa-Fluor 555 anti-mouse IgG Invitrogen . The image was taken on a BD Pathway 855 Bioimager using a 20x objective. This antibody also stained A549 ATCC CCL-185 and U-2 OS ATCC HTB-96 cells. The recommended permeabilization agent is Perm Buffer III see recommended assay procedure . Left Figure: Western blot analysis of Stat1 on a A431 lysate. Lane 1: 1:1000, lane 2: 1:2000, lane 3: 1:4000 dilution of the anti-Stat1 antibody. Right Figure: Immunofluorescent staining of HeLa ATCC CCL-2 cells. Cells were seeded in a 96 well imaging plate Cat. No. 353219 at 10,000 cells per well. After overnight incubation, cells were either mock treated media, left or exposed to IFN-gamma 100 ng/ml, right for 15 minutes. After treatment, cells were stained using the alcohol perm protocol and the anti-Stat1 antibody. The second step reagent was Alexa-Fluor 555 anti-mouse IgG Invitrogen . The image was taken on a BD Pathway 855 Bioimager using a 20x objective. This antibody also stained A549 ATCC CCL-185 and U-2 OS ATCC HTB-96 cells. The recommended permeabilization agent is Perm Buffer III see recommended assay procedure .