BD Transduction LaboratoriesTM Purified Mouse Anti-Stat1 (pY701)

Description

Western blot analysis for Stat1 pY701 far left figure . A431 cells Human epithelial carcinoma; ATCC CRL-1555 were either left untreated lane 1 or treated with 100 ng/ml EGF for 5 minutes at 37C lane 2 . The top panel was probed with a mouse anti-Stat1 antibody Cat. No. 610115 while the bottom panel was probed with the mouse anti-Stat1 pY701 antibody at a 1:1000 dilution. Immunofluorescence staining for Stat1 pY701 middle left figure . A431 cells Human epithelial carcinoma; ATCC CRL-1555 were either untreated top left and bottom left quadrants or were serum starved and then treated with 100 ng/ml EGF for 5 minutes, then fixed in 3.75 % paraformaldehyde with 0.2 % Trtion-X 100 top right and bottom right quandrants . Immunofluorescent staining was performed with a mouse anti-Stat1 antibody Cat. No. 610115 top left and top right quadrants and the mouse anti-Stat1 pY701 antibody bottom left and bottom right quadrants . Flow cytometric staining for Stat1 pY701 middle right and far right figures . U-937 cells Human histiocytic lymphoma; ATCC CRL-1593.2 were either untreated unshaded histograms or serum starved overnight and treated with 1000 units/mL of IFN-gamma for 15 min shaded histograms . Cells were fixed with 1 % formaldehyde, followed by 80 % ethanol and BD Cytofix/Cytoperm Cat. No. 554714 . Cells were then stained with a mouse anti-Stat1 antibody Cat. No. 610185 middle right figure or the mouse anti-Stat1 pY701 antibody far right figure . Western blot analysis for Stat1 pY701 far left figure . A431 cells Human epithelial carcinoma; ATCC CRL-1555 were either left untreated lane 1 or treated with 100 ng/ml EGF for 5 minutes at 37C lane 2 . The top panel was probed with a mouse anti-Stat1 antibody Cat. No. 610115 while the bottom panel was probed with the mouse anti-Stat1 pY701 antibody at a 1:1000 dilution. Immunofluorescence staining for Stat1 pY701 middle left figure . A431 cells Human epithelial carcinoma; ATCC CRL-1555 were either untreated top left and bottom left quadrants or were serum starved and then treated with 100 ng/ml EGF for 5 minutes, then fixed in 3.75 % paraformaldehyde with 0.2 % Trtion-X 100 top right and bottom right quandrants . Immunofluorescent staining was performed with a mouse anti-Stat1 antibody Cat. No. 610115 top left and top right quadrants and the mouse anti-Stat1 pY701 antibody bottom left and bottom right quadrants . Flow cytometric staining for Stat1 pY701 middle right and far right figures . U-937 cells Human histiocytic lymphoma; ATCC CRL-1593.2 were either untreated unshaded histograms or serum starved overnight and treated with 1000 units/mL of IFN-gamma for 15 min shaded histograms . Cells were fixed with 1 % formaldehyde, followed by 80 % ethanol and BD Cytofix/Cytoperm Cat. No. 554714 . Cells were then stained with a mouse anti-Stat1 antibody Cat. No. 610185 middle right figure or the mouse anti-Stat1 pY701 antibody far right figure . Show More Western blot analysis for Stat1 pY701 far left figure . A431 cells Human epithelial carcinoma; ATCC CRL-1555 were either left untreated lane 1 or treated with 100 ng/ml EGF for 5 minutes at 37C lane 2 . The top panel was probed with a mouse anti-Stat1 antibody Cat. No. 610115 while the bottom panel was probed with the mouse anti-Stat1 pY701 antibody at a 1:1000 dilution. Immunofluorescence staining for Stat1 pY701 middle left figure . A431 cells Human epithelial carcinoma; ATCC CRL-1555 were either untreated top left and bottom left quadrants or were serum starved and then treated with 100 ng/ml EGF for 5 minutes, then fixed in 3.75 % paraformaldehyde with 0.2 % Trtion-X 100 top right and bottom right quandrants . Immunofluorescent staining was performed with a mouse anti-Stat1 antibody Cat. No. 610115 top left and top right quadrants and the mouse anti-Stat1 pY701 antibody bottom left and bottom right quadrants . Flow cytometric staining for Stat1 pY701 middle right and far right figures . U-937 cells Human histiocytic lymphoma; ATCC CRL-1593.2 were either untreated unshaded histograms or serum starved overnight and treated with 1000 units/mL of IFN-gamma for 15 min shaded histograms . Cells were fixed with 1 % formaldehyde, followed by 80 % ethanol and BD Cytofix/Cytoperm Cat. No. 554714 . Cells were then stained with a mouse anti-Stat1 antibody Cat. No. 610185 middle right figure or the mouse anti-Stat1 pY701 antibody far right figure .