BD Transduction LaboratoriesTM Purified Mouse Anti-Synaptophysin

Description

Western blot analysis of Synaptophysin on a rat cerebrum lysate left . Lane 1: 1:25,000, lane 2: 1:50,000, lane 3: 1:100,000 dilution of the anti- synaptophysin antibody. Immunofluorescent staining of SK-N-SH cells right . Cells were seeded in a 384 well collagen coated Microplates Material # 353962 at 8,000 cells per well. After overnight incubation, cells were stained using the methanol fix/perm protocol see Recommended Assay Procedure; Bioimaging protocol link and the anti- Synaptophysin antibody. The second step reagent was Alexa Fluor 488 goat anti mouse Ig Invitrogen pseudo colored green . Cell nuclei were counter stained with Hoechst 33342 pseudo colored blue . The image was taken on a BD Pathway 855 or 435 Bioimager System using a 20x objective and merged using the BD AttoVison software. This antibody also stained SH-SY5Y cells using both the Triton X100 and methanol fix/perm protocols see Recommended Assay Procedure; Bioimaging protocol link . Western blot analysis of Synaptophysin on a rat cerebrum lysate left . Lane 1: 1:25,000, lane 2: 1:50,000, lane 3: 1:100,000 dilution of the anti- synaptophysin antibody. Immunofluorescent staining of SK-N-SH cells right . Cells were seeded in a 384 well collagen coated Microplates Material # 353962 at 8,000 cells per well. After overnight incubation, cells were stained using the methanol fix/perm protocol see Recommended Assay Procedure; Bioimaging protocol link and the anti- Synaptophysin antibody. The second step reagent was Alexa Fluor 488 goat anti mouse Ig Invitrogen pseudo colored green . Cell nuclei were counter stained with Hoechst 33342 pseudo colored blue . The image was taken on a BD Pathway 855 or 435 Bioimager System using a 20x objective and merged using the BD AttoVison software. This antibody also stained SH-SY5Y cells using both the Triton X100 and methanol fix/perm protocols see Recommended Assay Procedure; Bioimaging protocol link . Western blot analysis of Synaptophysin on a rat cerebrum lysate left . Lane 1: 1:25,000, lane 2: 1:50,000, lane 3: 1:100,000 dilution of the anti- synaptophysin antibody. Immunofluorescent staining of SK-N-SH cells right . Cells were seeded in a 384 well collagen coated Microplates Material # 353962 at 8,000 cells per well. After overnight incubation, cells were stained using the methanol fix/perm protocol see Recommended Assay Procedure; Bioimaging protocol link and the anti- Synaptophysin antibody. The second step reagent was Alexa Fluor 488 goat anti mouse Ig Invitrogen pseudo colored green . Cell nuclei were counter stained with Hoechst 33342 pseudo colored blue . The image was taken on a BD Pathway 855 or 435 Bioimager System using a 20x objective and merged using the BD AttoVison software. This antibody also stained SH-SY5Y cells using both the Triton X100 and methanol fix/perm protocols see Recommended Assay Procedure; Bioimaging protocol link . Show More Western blot analysis of Synaptophysin on a rat cerebrum lysate left . Lane 1: 1:25,000, lane 2: 1:50,000, lane 3: 1:100,000 dilution of the anti- synaptophysin antibody. Immunofluorescent staining of SK-N-SH cells right . Cells were seeded in a 384 well collagen coated Microplates Material # 353962 at 8,000 cells per well. After overnight incubation, cells were stained using the methanol fix/perm protocol see Recommended Assay Procedure; Bioimaging protocol link and the anti- Synaptophysin antibody. The second step reagent was Alexa Fluor 488 goat anti mouse Ig Invitrogen pseudo colored green . Cell nuclei were counter stained with Hoechst 33342 pseudo colored blue . The image was taken on a BD Pathway 855 or 435 Bioimager System using a 20x objective and merged using the BD AttoVison software. This antibody also stained SH-SY5Y cells using both the Triton X100 and methanol fix/perm protocols see Recommended Assay Procedure; Bioimaging protocol link . Show More Western blot analysis of Synaptophysin on a rat cerebrum lysate left . Lane 1: 1:25,000, lane 2: 1:50,000, lane 3: 1:100,000 dilution of the anti- synaptophysin antibody. Immunofluorescent staining of SK-N-SH cells right . Cells were seeded in a 384 well collagen coated Microplates Material # 353962 at 8,000 cells per well. After overnight incubation, cells were stained using the methanol fix/perm protocol see Recommended Assay Procedure; Bioimaging protocol link and the anti- Synaptophysin antibody. The second step reagent was Alexa Fluor 488 goat anti mouse Ig Invitrogen pseudo colored green . Cell nuclei were counter stained with Hoechst 33342 pseudo colored blue . The image was taken on a BD Pathway 855 or 435 Bioimager System using a 20x objective and merged using the BD AttoVison software. This antibody also stained SH-SY5Y cells using both the Triton X100 and methanol fix/perm protocols see Recommended Assay Procedure; Bioimaging protocol link . Western blot analysis of Synaptophysin on a rat cerebrum lysate left . Lane 1: 1:25,000, lane 2: 1:50,000, lane 3: 1:100,000 dilution of the anti- synaptophysin antibody. Immunofluorescent staining of SK-N-SH cells right . Cells were seeded in a 384 well collagen coated Microplates Material # 353962 at 8,000 cells per well. After overnight incubation, cells were stained using the methanol fix/perm protocol see Recommended Assay Procedure; Bioimaging protocol link and the anti- Synaptophysin antibody. The second step reagent was Alexa Fluor 488 goat anti mouse Ig Invitrogen pseudo colored green . Cell nuclei were counter stained with Hoechst 33342 pseudo colored blue . The image was taken on a BD Pathway 855 or 435 Bioimager System using a 20x objective and merged using the BD AttoVison software. This antibody also stained SH-SY5Y cells using both the Triton X100 and methanol fix/perm protocols see Recommended Assay Procedure; Bioimaging protocol link .