BDTM AccutaseTM Cell Detachment Solution

Description

Human Embryonic Stem ES cells detached with Accutase and analyzed with cell surface markers of pluripotency. H9 ES cells WiCell, Madison, WI that were grown on BD Matrigel hESC-qualified Matrix Cat. No. 354277 in mTeSR 1 medium Stem Cell Technologies were detached with Accutase Cell Detachment Solution. The cells were stained with either PE Mouse anti-SSEA-4 Cat. 560128 and Alexa Fluor 647 Mouse anti-Human TRA-1-81 Cat. 560793 or PE Mouse IgG3, kappa Isotype Control Cat. 556659 and Alexa Fluor 647 Mouse IgM, kappa Isotype Control Cat. 560806 and then analyzed with Diamidino-2-phenylindole dihydrochloride DAPI Sigma, Cat. No. D-9542 for live/dead cell discrimination. Flow cytometry was performed on a BD LSR II flow cytometry system. For data analysis, live cells were first gated left panel , and then single cells were selected by light scatter gating center panel . Expression of surface pluripotency markers was then determined right panel , with the positions of the quadrant markers based upon the isotype controls data not shown . Human Embryonic Stem ES cells detached with Accutase and analyzed with cell surface markers of pluripotency. H9 ES cells WiCell, Madison, WI that were grown on BD Matrigel hESC-qualified Matrix Cat. No. 354277 in mTeSR 1 medium Stem Cell Technologies were detached with Accutase Cell Detachment Solution. The cells were stained with either PE Mouse anti-SSEA-4 Cat. 560128 and Alexa Fluor 647 Mouse anti-Human TRA-1-81 Cat. 560793 or PE Mouse IgG3, kappa Isotype Control Cat. 556659 and Alexa Fluor 647 Mouse IgM, kappa Isotype Control Cat. 560806 and then analyzed with Diamidino-2-phenylindole dihydrochloride DAPI Sigma, Cat. No. D-9542 for live/dead cell discrimination. Flow cytometry was performed on a BD LSR II flow cytometry system. For data analysis, live cells were first gated left panel , and then single cells were selected by light scatter gating center panel . Expression of surface pluripotency markers was then determined right panel , with the positions of the quadrant markers based upon the isotype controls data not shown . Human Embryonic Stem ES cells detached with Accutase and analyzed with cell surface markers of pluripotency. H9 ES cells WiCell, Madison, WI that were grown on BD Matrigel hESC-qualified Matrix Cat. No. 354277 in mTeSR 1 medium Stem Cell Technologies were detached with Accutase Cell Detachment Solution. The cells were stained with either PE Mouse anti-SSEA-4 Cat. 560128 and Alexa Fluor 647 Mouse anti-Human TRA-1-81 Cat. 560793 or PE Mouse IgG3, kappa Isotype Control Cat. 556659 and Alexa Fluor 647 Mouse IgM, kappa Isotype Control Cat. 560806 and then analyzed with Diamidino-2-phenylindole dihydrochloride DAPI Sigma, Cat. No. D-9542 for live/dead cell discrimination. Flow cytometry was performed on a BD LSR II flow cytometry system. For data analysis, live cells were first gated left panel , and then single cells were selected by light scatter gating center panel . Expression of surface pluripotency markers was then determined right panel , with the positions of the quadrant markers based upon the isotype controls data not shown . Show More Human Embryonic Stem ES cells detached with Accutase and analyzed with cell surface markers of pluripotency. H9 ES cells WiCell, Madison, WI that were grown on BD Matrigel hESC-qualified Matrix Cat. No. 354277 in mTeSR 1 medium Stem Cell Technologies were detached with Accutase Cell Detachment Solution. The cells were stained with either PE Mouse anti-SSEA-4 Cat. 560128 and Alexa Fluor 647 Mouse anti-Human TRA-1-81 Cat. 560793 or PE Mouse IgG3, kappa Isotype Control Cat. 556659 and Alexa Fluor 647 Mouse IgM, kappa Isotype Control Cat. 560806 and then analyzed with Diamidino-2-phenylindole dihydrochloride DAPI Sigma, Cat. No. D-9542 for live/dead cell discrimination. Flow cytometry was performed on a BD LSR II flow cytometry system. For data analysis, live cells were first gated left panel , and then single cells were selected by light scatter gating center panel . Expression of surface pluripotency markers was then determined right panel , with the positions of the quadrant markers based upon the isotype controls data not shown . Show More Human Embryonic Stem ES cells detached with Accutase and analyzed with cell surface markers of pluripotency. H9 ES cells WiCell, Madison, WI that were grown on BD Matrigel hESC-qualified Matrix Cat. No. 354277 in mTeSR 1 medium Stem Cell Technologies were detached with Accutase Cell Detachment Solution. The cells were stained with either PE Mouse anti-SSEA-4 Cat. 560128 and Alexa Fluor 647 Mouse anti-Human TRA-1-81 Cat. 560793 or PE Mouse IgG3, kappa Isotype Control Cat. 556659 and Alexa Fluor 647 Mouse IgM, kappa Isotype Control Cat. 560806 and then analyzed with Diamidino-2-phenylindole dihydrochloride DAPI Sigma, Cat. No. D-9542 for live/dead cell discrimination. Flow cytometry was performed on a BD LSR II flow cytometry system. For data analysis, live cells were first gated left panel , and then single cells were selected by light scatter gating center panel . Expression of surface pluripotency markers was then determined right panel , with the positions of the quadrant markers based upon the isotype controls data not shown . Human Embryonic Stem ES cells detached with Accutase and analyzed with cell surface markers of pluripotency. H9 ES cells WiCell, Madison, WI that were grown on BD Matrigel hESC-qualified Matrix Cat. No. 354277 in mTeSR 1 medium Stem Cell Technologies were detached with Accutase Cell Detachment Solution. The cells were stained with either PE Mouse anti-SSEA-4 Cat. 560128 and Alexa Fluor 647 Mouse anti-Human TRA-1-81 Cat. 560793 or PE Mouse IgG3, kappa Isotype Control Cat. 556659 and Alexa Fluor 647 Mouse IgM, kappa Isotype Control Cat. 560806 and then analyzed with Diamidino-2-phenylindole dihydrochloride DAPI Sigma, Cat. No. D-9542 for live/dead cell discrimination. Flow cytometry was performed on a BD LSR II flow cytometry system. For data analysis, live cells were first gated left panel , and then single cells were selected by light scatter gating center panel . Expression of surface pluripotency markers was then determined right panel , with the positions of the quadrant markers based upon the isotype controls data not shown .