BDTM BV421 BCMA CAR Detection Reagent

Description

Detection of BCMA CAR expression on transfected CHO cells. CHO cells were transfected with a CAR analog construct, comprised of cell-surface Anti-Human BCMA scFv, an epitope tag, and a transmembrane domain. The cells were then stained with BD BV421 BCMA CAR Detection Reagent Cat. No. 572806/572619 and Alexa Fluor 647 epitope tag-specific antibody for 30 minutes at room temperature. BD Via-Probe Cell Viability 7-AAD Solution Cat. No. 555815 was added to cells right before analysis. The bivariate pseudocolor density plot shows BCMA CAR expression correlated with epitope tag-specific staining, gated on viable 7-AAD-negative CHO cells. Flow cytometry was performed using a BD LSRFortessa Cell Analyzer System and FlowJo Software. Detection of BCMA CAR expression on transduced primary Human T cells. Human T cells from a healthy donor were purified using the BD IMag Human T Lymphocyte Enrichment Set-DM Cat. No. 557874 . T cells were then activated with Anti-CD3/CD28 antibody-coupled beads plus purified BD Pharmingen Recombinant Human IL-2 protein Cat. No. 554603 for 2 days, infected with BCMA CAR lentivirus, expanded for 7-10 days in culture with IL-2, and cryopreserved. Thawed BCMA CAR-transduced cells or mock transduced cells were stained with BD BV421 BCMA CAR Detection Reagent Cat. No. 572806/572619 for 30 minutes at room temperature. BD Via-Probe Cell Viability 7-AAD Solution Cat. No. 555815 was added to cells right before analysis. Overlapping histograms show BCMA CAR staining with BD BV421 BCMA CAR Detection Reagent of either BCMA CAR-transduced cells solid line versus mock transduced cells dashed line , gated on viable 7-AAD-negative cells. Flow cytometry was performed using a BD LSRFortessa Cell Analyzer System and FlowJo Software. Detection of BCMA CAR expression on transfected CHO cells. CHO cells were transfected with a CAR analog construct, comprised of cell-surface Anti-Human BCMA scFv, an epitope tag, and a transmembrane domain. The cells were then stained with BD BV421 BCMA CAR Detection Reagent Cat. No. 572806/572619 and Alexa Fluor 647 epitope tag-specific antibody for 30 minutes at room temperature. BD Via-Probe Cell Viability 7-AAD Solution Cat. No. 555815 was added to cells right before analysis. The bivariate pseudocolor density plot shows BCMA CAR expression correlated with epitope tag-specific staining, gated on viable 7-AAD-negative CHO cells. Flow cytometry was performed using a BD LSRFortessa Cell Analyzer System and FlowJo Software. Show More Detection of BCMA CAR expression on transduced primary Human T cells. Human T cells from a healthy donor were purified using the BD IMag Human T Lymphocyte Enrichment Set-DM Cat. No. 557874 . T cells were then activated with Anti-CD3/CD28 antibody-coupled beads plus purified BD Pharmingen Recombinant Human IL-2 protein Cat. No. 554603 for 2 days, infected with BCMA CAR lentivirus, expanded for 7-10 days in culture with IL-2, and cryopreserved. Thawed BCMA CAR-transduced cells or mock transduced cells were stained with BD BV421 BCMA CAR Detection Reagent Cat. No. 572806/572619 for 30 minutes at room temperature. BD Via-Probe Cell Viability 7-AAD Solution Cat. No. 555815 was added to cells right before analysis. Overlapping histograms show BCMA CAR staining with BD BV421 BCMA CAR Detection Reagent of either BCMA CAR-transduced cells solid line versus mock transduced cells dashed line , gated on viable 7-AAD-negative cells. Flow cytometry was performed using a BD LSRFortessa Cell Analyzer System and FlowJo Software. Show More Detection of BCMA CAR expression on transfected CHO cells. CHO cells were transfected with a CAR analog construct, comprised of cell-surface Anti-Human BCMA scFv, an epitope tag, and a transmembrane domain. The cells were then stained with BD BV421 BCMA CAR Detection Reagent Cat. No. 572806/572619 and Alexa Fluor 647 epitope tag-specific antibody for 30 minutes at room temperature. BD Via-Probe Cell Viability 7-AAD Solution Cat. No. 555815 was added to cells right before analysis. The bivariate pseudocolor density plot shows BCMA CAR expression correlated with epitope tag-specific staining, gated on viable 7-AAD-negative CHO cells. Flow cytometry was performed using a BD LSRFortessa Cell Analyzer System and FlowJo Software. Detection of BCMA CAR expression on transduced primary Human T cells. Human T cells from a healthy donor were purified using the BD IMag Human T Lymphocyte Enrichment Set-DM Cat. No. 557874 . T cells were then activated with Anti-CD3/CD28 antibody-coupled beads plus purified BD Pharmingen Recombinant Human IL-2 protein Cat. No. 554603 for 2 days, infected with BCMA CAR lentivirus, expanded for 7-10 days in culture with IL-2, and cryopreserved. Thawed BCMA CAR-transduced cells or mock transduced cells were stained with BD BV421 BCMA CAR Detection Reagent Cat. No. 572806/572619 for 30 minutes at room temperature. BD Via-Probe Cell Viability 7-AAD Solution Cat. No. 555815 was added to cells right before analysis. Overlapping histograms show BCMA CAR staining with BD BV421 BCMA CAR Detection Reagent of either BCMA CAR-transduced cells solid line versus mock transduced cells dashed line , gated on viable 7-AAD-negative cells. Flow cytometry was performed using a BD LSRFortessa Cell Analyzer System and FlowJo Software.