BDTM Cytometric Bead Array (CBA) Human Soluble CD54 (ICAM-1) Flex Set

Description

Figure 1. Example BD CBA Human sCD54 ICAM-1 Flex Set standard curve. Data acquired on a BD FACSArray bioanalyzer and analyzed using FCAP Array Software. Reproducibility: The intra-assay and inter-assay reproducibility were determined for the BD CBA Human sCD54 ICAM-1 Flex Set by evaluating ten replicates of three different sample levels intra-assay and three replicates of three different sample levels from four separate experiments inter-assay . Recovery: Cell culture supernatant samples were spiked with three different levels of protein. The spiked samples were assayed and the results were compared with expected values. ND = Not Determined. Due to high levels of sCD54 in serum and EDTA-plasma samples, recovery information could not be generated. Linearity: Cell culture supernatant samples were spiked with protein and serially diluted. The diluted samples were assayed and the results were compared with the original spiked sample. ND = Not Determined. Due to high levels of sCD54 in serum and EDTA-plasma samples, linearity information could not be generated. Figure 1. Example BD CBA Human sCD54 ICAM-1 Flex Set standard curve. Data acquired on a BD FACSArray bioanalyzer and analyzed using FCAP Array Software. Show More Reproducibility: The intra-assay and inter-assay reproducibility were determined for the BD CBA Human sCD54 ICAM-1 Flex Set by evaluating ten replicates of three different sample levels intra-assay and three replicates of three different sample levels from four separate experiments inter-assay . Show More Recovery: Cell culture supernatant samples were spiked with three different levels of protein. The spiked samples were assayed and the results were compared with expected values. ND = Not Determined. Due to high levels of sCD54 in serum and EDTA-plasma samples, recovery information could not be generated. Show More Linearity: Cell culture supernatant samples were spiked with protein and serially diluted. The diluted samples were assayed and the results were compared with the original spiked sample. ND = Not Determined. Due to high levels of sCD54 in serum and EDTA-plasma samples, linearity information could not be generated. Show More Figure 1. Example BD CBA Human sCD54 ICAM-1 Flex Set standard curve. Data acquired on a BD FACSArray bioanalyzer and analyzed using FCAP Array Software. Reproducibility: The intra-assay and inter-assay reproducibility were determined for the BD CBA Human sCD54 ICAM-1 Flex Set by evaluating ten replicates of three different sample levels intra-assay and three replicates of three different sample levels from four separate experiments inter-assay . Recovery: Cell culture supernatant samples were spiked with three different levels of protein. The spiked samples were assayed and the results were compared with expected values. ND = Not Determined. Due to high levels of sCD54 in serum and EDTA-plasma samples, recovery information could not be generated. Linearity: Cell culture supernatant samples were spiked with protein and serially diluted. The diluted samples were assayed and the results were compared with the original spiked sample. ND = Not Determined. Due to high levels of sCD54 in serum and EDTA-plasma samples, linearity information could not be generated.