BDTM Cytometric Bead Array (CBA) Human Soluble CD62E (E-Selectin) Flex Set

Description

Figure 1. Example BD CBA Human Soluble CD62E E-Selectin Flex Set standard curve. Data acquired on a BD FACSArray bioanalyzer and analyzed using FCAP Array Software. Linearity: Cell culture supernatant, 1:4 diluted serum, or 1:4 diluted EDTA-treated plasma were spiked with protein and serially diluted. The diluted samples were assayed and the results were compared with the original spiked sample. ND = Not Determined. Due to high levels of CD62E E-Selectin in serum and EDTA-plasma samples, linearity information could not be generated. Recovery: Cell culture supernatant, serum, or EDTA-treated plasma were spiked with three different levels of protein. The spiked samples were assayed and the results were compared with expected values. Serum and plasma samples were diluted 1:4 before the protein was spiked into each. Serum is a pool of 800 - 1000 donors and the plasma was pooled from at least 20 donors. ND = Not Determined. Due to high levels of sCD62E E-Selectin in serum and EDTA-plasma samples, recovery information could not be generated. Reproducibility: The intra-assay and inter-assay reproducibility were determined for the BD CBA Human Soluble CD62E E-Selectin Flex Set by evaluating ten replicates of three different sample levels intra-assay and three replicates of three different sample levels from four separate experiments inter-assay . Figure 1. Example BD CBA Human Soluble CD62E E-Selectin Flex Set standard curve. Data acquired on a BD FACSArray bioanalyzer and analyzed using FCAP Array Software. Show More Linearity: Cell culture supernatant, 1:4 diluted serum, or 1:4 diluted EDTA-treated plasma were spiked with protein and serially diluted. The diluted samples were assayed and the results were compared with the original spiked sample. ND = Not Determined. Due to high levels of CD62E E-Selectin in serum and EDTA-plasma samples, linearity information could not be generated. Show More Recovery: Cell culture supernatant, serum, or EDTA-treated plasma were spiked with three different levels of protein. The spiked samples were assayed and the results were compared with expected values. Serum and plasma samples were diluted 1:4 before the protein was spiked into each. Serum is a pool of 800 - 1000 donors and the plasma was pooled from at least 20 donors. ND = Not Determined. Due to high levels of sCD62E E-Selectin in serum and EDTA-plasma samples, recovery information could not be generated. Show More Reproducibility: The intra-assay and inter-assay reproducibility were determined for the BD CBA Human Soluble CD62E E-Selectin Flex Set by evaluating ten replicates of three different sample levels intra-assay and three replicates of three different sample levels from four separate experiments inter-assay . Show More Figure 1. Example BD CBA Human Soluble CD62E E-Selectin Flex Set standard curve. Data acquired on a BD FACSArray bioanalyzer and analyzed using FCAP Array Software. Linearity: Cell culture supernatant, 1:4 diluted serum, or 1:4 diluted EDTA-treated plasma were spiked with protein and serially diluted. The diluted samples were assayed and the results were compared with the original spiked sample. ND = Not Determined. Due to high levels of CD62E E-Selectin in serum and EDTA-plasma samples, linearity information could not be generated. Recovery: Cell culture supernatant, serum, or EDTA-treated plasma were spiked with three different levels of protein. The spiked samples were assayed and the results were compared with expected values. Serum and plasma samples were diluted 1:4 before the protein was spiked into each. Serum is a pool of 800 - 1000 donors and the plasma was pooled from at least 20 donors. ND = Not Determined. Due to high levels of sCD62E E-Selectin in serum and EDTA-plasma samples, recovery information could not be generated. Reproducibility: The intra-assay and inter-assay reproducibility were determined for the BD CBA Human Soluble CD62E E-Selectin Flex Set by evaluating ten replicates of three different sample levels intra-assay and three replicates of three different sample levels from four separate experiments inter-assay .