BDTM DimerX DimerX I: PE Soluble Dimeric Mouse H-2Kb:Ig Fusion Protein

Description

Schematic representation of the MHC class I:Ig dimeric protein. Antigen-specific T-cell identification of OT-1 TCR transgenic mouse splenocytes. Splenocytes from OT-1 TCR transgenic mice were incubated with Mouse BD Fc Block purified anti-mouse CD16/CD32 mAb 2.4G2 Cat. No. 553141/553142, both panels and stained with FITC-conjugated anti-mouse CD8a mAb 53-6.7 Cat. No. 553030/553031, both panels and PE-conjugated DimerX H-2Kb-:Ig Fusion Protein both panels loaded overnight at 4C with OVA peptide left panel or SIY peptide right panel . Data shown is for viable cells only 10 % probability plot . Cells were gated based on propidium iodide staining, as well as light-scatter characteristics. Data was acquired on a BD FACSCalibur flow cytometry system. Schematic representation of the MHC class I:Ig dimeric protein. Show More Antigen-specific T-cell identification of OT-1 TCR transgenic mouse splenocytes. Splenocytes from OT-1 TCR transgenic mice were incubated with Mouse BD Fc Block purified anti-mouse CD16/CD32 mAb 2.4G2 Cat. No. 553141/553142, both panels and stained with FITC-conjugated anti-mouse CD8a mAb 53-6.7 Cat. No. 553030/553031, both panels and PE-conjugated DimerX H-2Kb-:Ig Fusion Protein both panels loaded overnight at 4C with OVA peptide left panel or SIY peptide right panel . Data shown is for viable cells only 10 % probability plot . Cells were gated based on propidium iodide staining, as well as light-scatter characteristics. Data was acquired on a BD FACSCalibur flow cytometry system. Show More Schematic representation of the MHC class I:Ig dimeric protein. Antigen-specific T-cell identification of OT-1 TCR transgenic mouse splenocytes. Splenocytes from OT-1 TCR transgenic mice were incubated with Mouse BD Fc Block purified anti-mouse CD16/CD32 mAb 2.4G2 Cat. No. 553141/553142, both panels and stained with FITC-conjugated anti-mouse CD8a mAb 53-6.7 Cat. No. 553030/553031, both panels and PE-conjugated DimerX H-2Kb-:Ig Fusion Protein both panels loaded overnight at 4C with OVA peptide left panel or SIY peptide right panel . Data shown is for viable cells only 10 % probability plot . Cells were gated based on propidium iodide staining, as well as light-scatter characteristics. Data was acquired on a BD FACSCalibur flow cytometry system.