BDTM DimerX DimerX I: Recombinant Soluble Dimeric Mouse H-2D[b]:Ig
551323 ·
BDB Bioscience · Cat: 551323
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Schematic representation of the MHC class I:Ig dimeric protein. Flow cytometric analysis of a T-cell hybridoma using DimerX I H-2Db:Ig. Mouse DimerX I H-2Db:Ig was incubated with a 160-molar excess of a specific peptide MOG 44-54 FSRVVHLYRNG, Panel A or irrelevant peptide Influenza A34 NP 366-374 ASNENMETM, Panel B at 37C overnight. Peptide-loaded H-2Db:Ig was then used for the immunofluorescent staining of MOG TH10 hybridoma cells, along with FITC conjugated anti-mouse CD3e mAb 145-2C11 Cat. no. 553061/553062 followed by PE-conjugated anti-mouse IgG1 mAb A85-1 Cat. no. 550083 . Flow cytometry was performed on a BD FACSCalibur flow cytometry system. Schematic representation of the MHC class I:Ig dimeric protein. Show More Flow cytometric analysis of a T-cell hybridoma using DimerX I H-2Db:Ig. Mouse DimerX I H-2Db:Ig was incubated with a 160-molar excess of a specific peptide MOG 44-54 FSRVVHLYRNG, Panel A or irrelevant peptide Influenza A34 NP 366-374 ASNENMETM, Panel B at 37C overnight. Peptide-loaded H-2Db:Ig was then used for the immunofluorescent staining of MOG TH10 hybridoma cells, along with FITC conjugated anti-mouse CD3e mAb 145-2C11 Cat. no. 553061/553062 followed by PE-conjugated anti-mouse IgG1 mAb A85-1 Cat. no. 550083 . Flow cytometry was performed on a BD FACSCalibur flow cytometry system. Show More Schematic representation of the MHC class I:Ig dimeric protein. Flow cytometric analysis of a T-cell hybridoma using DimerX I H-2Db:Ig. Mouse DimerX I H-2Db:Ig was incubated with a 160-molar excess of a specific peptide MOG 44-54 FSRVVHLYRNG, Panel A or irrelevant peptide Influenza A34 NP 366-374 ASNENMETM, Panel B at 37C overnight. Peptide-loaded H-2Db:Ig was then used for the immunofluorescent staining of MOG TH10 hybridoma cells, along with FITC conjugated anti-mouse CD3e mAb 145-2C11 Cat. no. 553061/553062 followed by PE-conjugated anti-mouse IgG1 mAb A85-1 Cat. no. 550083 . Flow cytometry was performed on a BD FACSCalibur flow cytometry system.