BDTM SpectraCompTM Unmixing and Compensation Particles

Description

Figure 1. BD SpectraComp Unmixing and Compensation Particles were labeled with PE-conjugated monoclonal antibodies of different Ig isotypes from different species at 0.25 mug or 0.5 mug for each clone following the recommended protocol. The PE YG585 fluorescence histograms show the staining profiles for either unstained particles lowest fluorescence or for particles labeled with one staining monoclonal antibody of mouse, rat, or hamster origin. The emitted fluorescence of the particles was excited by 561 nm yellow-green laser and captured by YG 585 nm detector. Flow cytometry and data analysis were performed using a BD FACSymphony A5 SE Cell Analyzer System and FlowJo Software. Figure 2. Human whole blood sample Left Plot and BD SpectraComp Unmixing and Compensation Particles sample Right Plot were treated with BD Pharm Lyse Lysing Buffer Cat. No. 555899 , then washed with BD Pharmingen Stain Buffer FBS Cat. No. 554656 . Data were acquired under the same forward and side-light scatter settings, ie, using the same voltages for FSC-A and SSC-A. Flow cytometry and data analysis were performed using a BD LSRFortessa Cell Analyzer System and FlowJo Software. Figure 3. Autofluorescence of BD SpectraComp Unmixing and Compensation Particles measured as Median Fluorescence Intensity MFI derived from the full spectrum detectors of a BD FACSymphony A5 SE Cell Analyzer System and FlowJo Software. Figure 1. BD SpectraComp Unmixing and Compensation Particles were labeled with PE-conjugated monoclonal antibodies of different Ig isotypes from different species at 0.25 mug or 0.5 mug for each clone following the recommended protocol. The PE YG585 fluorescence histograms show the staining profiles for either unstained particles lowest fluorescence or for particles labeled with one staining monoclonal antibody of mouse, rat, or hamster origin. The emitted fluorescence of the particles was excited by 561 nm yellow-green laser and captured by YG 585 nm detector. Flow cytometry and data analysis were performed using a BD FACSymphony A5 SE Cell Analyzer System and FlowJo Software. Show More Figure 2. Human whole blood sample Left Plot and BD SpectraComp Unmixing and Compensation Particles sample Right Plot were treated with BD Pharm Lyse Lysing Buffer Cat. No. 555899 , then washed with BD Pharmingen Stain Buffer FBS Cat. No. 554656 . Data were acquired under the same forward and side-light scatter settings, ie, using the same voltages for FSC-A and SSC-A. Flow cytometry and data analysis were performed using a BD LSRFortessa Cell Analyzer System and FlowJo Software. Figure 3. Autofluorescence of BD SpectraComp Unmixing and Compensation Particles measured as Median Fluorescence Intensity MFI derived from the full spectrum detectors of a BD FACSymphony A5 SE Cell Analyzer System and FlowJo Software. Show More Figure 1. BD SpectraComp Unmixing and Compensation Particles were labeled with PE-conjugated monoclonal antibodies of different Ig isotypes from different species at 0.25 mug or 0.5 mug for each clone following the recommended protocol. The PE YG585 fluorescence histograms show the staining profiles for either unstained particles lowest fluorescence or for particles labeled with one staining monoclonal antibody of mouse, rat, or hamster origin. The emitted fluorescence of the particles was excited by 561 nm yellow-green laser and captured by YG 585 nm detector. Flow cytometry and data analysis were performed using a BD FACSymphony A5 SE Cell Analyzer System and FlowJo Software. Figure 2. Human whole blood sample Left Plot and BD SpectraComp Unmixing and Compensation Particles sample Right Plot were treated with BD Pharm Lyse Lysing Buffer Cat. No. 555899 , then washed with BD Pharmingen Stain Buffer FBS Cat. No. 554656 . Data were acquired under the same forward and side-light scatter settings, ie, using the same voltages for FSC-A and SSC-A. Flow cytometry and data analysis were performed using a BD LSRFortessa Cell Analyzer System and FlowJo Software. Figure 3. Autofluorescence of BD SpectraComp Unmixing and Compensation Particles measured as Median Fluorescence Intensity MFI derived from the full spectrum detectors of a BD FACSymphony A5 SE Cell Analyzer System and FlowJo Software.