BlasTaqTM 2X qPCR MasterMix

Description

BlasTaq 2X qPCR MasterMix provides a convenient, reliable, and robust solution for performing quantitative real-time PCR qPCR analysis of DNA samples. This ready-to-use MasterMix contains BlasTaq DNA Polymerase, a next-generation Taq polymerase strategically engineered by abm to deliver faster extension rates and stronger overall performance. With optimized reaction conditions, BlasTaq DNA Polymerase offers significantly improved processivity, higher yields, and enhanced sensitivity. These improvements allow for faster reaction times, shortening qPCR runs by up to 70 % compared to reactions using wild-type Taq polymerase. BlasTaq possesses 5-3 polymerase activity and 5-3 exonuclease activity, but lacks 3-5 exonuclease activity. It also produces 3-dA-tailed amplicons, making qPCR products generated with BlasTaq compatible with TA cloning systems. The MasterMix contains a fluorescent dye compatible with commonly used detection systems, such as SYBR Green and EvaGreen. Additionally, ROX Reference Dye is supplied separately, ensuring compatibility with most qPCR instruments. For specific instrument requirements, please refer to the ROX Machine Compatibility Chart. Product Features: Enable streamlined protocol in a simple reaction set-up Allow accurate quantification of a variety of gene targets Reduce pipetting steps to minimize the risk of contamination Compatible with most real-time PCR instruments Contains dye comparable to SYBR Green and EvaGreen Probe-based qPCR Master Mix also available Included in abms PCR Buffet Program For a smaller size, see Cat. No. G891 500 rxn, 4 x 1.25 ml Product Component Quantity BlasTaq 2X qPCR MasterMix 2,500 rxn 25.0 ml ROX Reference Dye 240 mul ISO 13485:2016 MDSAP Certified Our PCR Products are manufactured under a Quality Management System conforming with ISO 13485:2016 as certified by Intertek a MDSAP recognized auditing organization .