BlasTaqTM HotStart 2X PCR MasterMix

Description

BlasTaq HotStart 2X PCR MasterMix is a convenient, ready-to-use solution containing abms BlasTaq HotStart DNA Polymerase in a specially formulated buffer with gel loading dye. This advanced Taq polymerase is engineered for rapid extension rates, robust performance, and includes a proprietary antibody that prevents the polymerase from working at low temperatures. The HotStart feature allows for easy reaction set-up at room temperature, preventing non-specific amplification and primer dimer formation. BlasTaq HotStart offers enhanced processivity, higher yields, increased sensitivity, and significantly reduced reaction timesup to 70 % faster than traditional wild-type Taq polymerase. During the initial denaturation, the antibody releases from the polymerase, restoring enzyme activity and reducing non-specific amplification, which ensures higher specificity and improved PCR product yields. BlasTaq also features 5-3 polymerase and exonuclease activities, but lacks 3-5 exonuclease activity, producing 3-dA-tailed amplicons that are compatible with TA cloning vectors. Product Features: No non-specific amplification or primer-dimers Improves yield of desired product Simplifies reaction setup Included in abms PCR Buffet Program ISO 13485:2016 MDSAP Certified Our PCR Products are manufactured under a Quality Management System conforming with ISO 13485:2016 as certified by Intertek a MDSAP recognized auditing organization .