BlasTaqTM HotStart DNA Polymerase

Description

Eliminate non-specific amplification and primer-dimer formation BlasTaq HotStart DNA Polymerase is a strategically-engineered, next generation Taq Polymerase that has rapid extension rates, robust performance, and contains a proprietary antibody that blocks polymerase activity at low temperatures. HotStart allows for a convenient reaction set-up at room temperature without non-specific amplification and primer dimer formation. With specialized reaction conditions, this polymerase provides increased processivity, yields, and sensitivity, while shortening reaction times by up to 70 %, compared to wild-type Taq DNA polymerase. During the initial denaturation step, the antibody dissociates from the DNA polymerase and restores enzyme activity. This feature significantly reduces non-specific product formation that would otherwise compete for reagent availability offering higher specificity and improved yield of PCR products. BlasTaq has 5-3 polymerase and 5-3 exonuclease activities, lacks 3-5 exonuclease activity, and produces 3-dA-tailed amplicons. PCR products made with BlasTaq can be used with TA cloning vectors. Product Features: No non-specific amplification or primer-dimers Improves yield of desired product Simplifies reaction setup Available as a MasterMix Cat. No. G598 Included in abms PCR Buffet Program Product Component Quantity BlasTaq HotStart DNA Polymerase 400 rxn 200 mul 5X BlasTaq Buffer 1 2 x 1.0 ml 1 Buffer contains 1.5 mM Mg2+. ISO 13485:2016 MDSAP Certified Our PCR Products are manufactured under a Quality Management System conforming with ISO 13485:2016 as certified by Intertek a MDSAP recognized auditing organization .