BlasTaqTM Probe 2X qPCR MasterMix

Description

BlasTaq Probe 2X qPCR MasterMix is designed for TaqMan probe-based real-time qPCR analysis of DNA samples. The components in the MasterMix including dNTPs, BlasTaq DNA polymerase, MgCl2, and other proprietary buffer components have been optimized to deliver superb sensitivity and performance in under one hour. BlasTaq is a next-generation, strategically engineered DNA polymerase that offers faster extension rates, improved processivity, and enhanced sensitivity compared to wild-type Taq polymerase. It possesses 5-3 polymerase activity and 5-3 exonuclease activity, which is essential for TaqMan probe hydrolysis during qPCR. It does not have 3-5 exonuclease proofreading activity, and produces 3-dA-tailed amplicons, which are compatible with TA cloning if needed. This MasterMix is suitable for a variety of applications, including SNP genotyping assays, gene expression analysis, microarray validation, and high-throughput screening. ROX reference dye is provided separately, ensuring universal compatibility with most qPCR instruments. Refer to the Rox Machine Compatibility Chart for instrument-specific guidelines. Product Features: Designed for TaqMan Probe-based qPCR Dye-based qPCR MasterMix also available 40-70 % less time than regular Taq 6 kb/min extension speed Highly robust and sensitive Probe-based One-Step RT-qPCR kit also available Cat. No. G596 Included in abms PCR Buffet Program Product Component Quantity BlasTaq Probe 2X qPCR MasterMix 500 rxn 4 x 1.25 ml ROX Reference Dye 50 mul ISO 13485:2016 MDSAP Certified Our PCR Products are manufactured under a Quality Management System conforming with ISO 13485:2016 as certified by Intertek a MDSAP recognized auditing organization .