BlasTaqTM Probe One-Step RT-qPCR

Description

BlasTaq Probe One-Step RT-qPCR combines reverse transcription and TaqMan probe-based real-time qPCR into a single-tube reaction for maximum convenience and efficiency. This system includes the RT-qPCR Enzyme Mix and BlasTaq Probe 2X qPCR MasterMix, providing highly sensitive and specific performance for both cDNA synthesis and real-time amplification. The proprietary RT-qPCR Enzyme Mix contains our next-generation BlasTaq DNA polymerase, along with specialized stabilizers and enhancers that optimize the two reactions in a seamless, single-step workflow. This design provides flexibility with a wide range of RNA templates and primer options, delivering a reliable, easy-to-use alternative to traditional two-step RT-qPCR. The MasterMix is suitable for a variety of applications, including SNP genotyping, gene expression analysis, microarray validation, and high-throughput screening. ROX reference dye is supplied separately, ensuring universal compatibility with most qPCR instruments. For instrument-specific guidelines, please refer to the ROX Machine Compatibility Chart. Product Features: Simple, one-step RT-qPCR reaction Flexibility with RNA templates and primer selection ROX reference dye provided separately Universally compatible with most qPCR instruments Included in abms PCR Buffet Program Product Component Quantity BlasTaq Probe 2X qPCR MasterMix 1.25 ml RT-qPCR Enzyme Mix 100 rxn 40 mul ROX Reference Dye 15 mul Nuclease-Free H2O 1.0 ml ISO 13485:2016 MDSAP Certified Our PCR Products are manufactured under a Quality Management System conforming with ISO 13485:2016 as certified by Intertek a MDSAP recognized auditing organization .