BORDETELLA SELECTIVE SUPPLEMENT

Description

Rehydrate the content of 1 vial aseptically with 2 ml of sterile distilled water. Mix well and aseptically add it to 500 ml of sterile, molten, cooled (45-50?C) B TM 044 – BORDET GENGOU AGAR BASE / TM 1147 – BORDET GENGOU AGAR BASE W/ 1.6% AGAR or 1000 ml of sterile, molten TM 063 – CHARCOAL AGAR BASE W/ NIACIN together with 10% v/v defibrinated horse blood. Mix well and pour into sterile petri plates. The vial content may be added to 500 ml of sterile half strength TM 062 – CHARCOAL AGAR BASE with 10% v/v defibrinated horse blood for use as a transport medium for Bordetella pertussis.