BRILLIANT GREEN AGAR MEDIUM 16. (as per IP)

Description

Brilliant Green Agar medium is recommended as a primary plating medium for isolation of Salmonella species was first described by Kristensen et al as medium for differentiation of paratyphoid B from other Gram negative enteric bacteria. Kauffmann further modified it for isolation of Salmonella from stool samples. Brilliant green agar is also recommended by APHA FDA and is in accordance with Indian Pharmacopoeia. This medium is employed in testing clinical specimens. Heavy inocula and heavily contaminated samples can be analyzed due to the outstanding selectivity of this medium. Brilliant Green Agar medium is used in the microbial limits test and with novobiocin for testing food and pharmaceutical products. Salmonella typhi, Shigella species, Escherichia coli, Proteus species, Pseudomonas species, Staphylococcus aureus are mostly inhibited. Being highly selective, it is recommended that this medium should be used along with a less inhibitory medium to increase the chances of recovery. Cultures are enriched in Selenite F-Broth or Tetrathionate Bile Brilliant green broth and plated on atleast two of the following selective media Brilliant Green Agar, Bismuth Sulphite Agar, Xylose Lysine Deoxycholate Agar and Deoxycholate Citrate Agar. Salmonella typhi and Shigella species may not grow on this medium, moreover Proteus, Pseudomonas and Citrobacter species may mimic enteric pathogens.