BYE AGAR

Description

Mycoplasmas (mollicutes) are the smallest free-living microorganisms. Earlier Mycoplasmataceae were given the general title of pleuropneumonia like organism (PPLO), because of similarities to Mycoplasma mycoides (subsp. mycoides), the causative agent of bovine pleuropneumonia. BYE media are simple media developed for cultivation and routine studies of distribution, habitat and possible pathogenesis of Mycoplasma – Pleuropneumonia like organisms and L-forms of bacteria by Barile, Yaguchi and Eveland. These media can be used for isolation of PPLOs from urethritis, penile ulcerations and cervical specimens and L-forms of Corynebacterium, Neisseria, and Streptococcus. These are also used for detecting PPLO contamination of tissue culture and cell-lines and for membrane filter work. Inoculations are made in duplicates. One set is incubated aerobically while the other anaerobically for 48 hours or more. Usually growth occurs within 3-5 days; however, negative results are reported after 10 days. Anaerobic conditions are most important for the first 3 days while secondary transfers can be incubated aerobically.