CapturemTM His-Tagged Purification Maxiprep Kit

Description

The Capturem His-Tagged Purification Maxiprep Kit provides single-use disposable membrane spin columns for simple, rapid purification of his-tagged proteins in up to 23 ml of clarified lysate. The columns are suitable for use under native or denaturing conditions, and in the presence of additives such as DTT up to 10 mM , betaME up to 30 mM , TCEP up to 5 mM , EDTA up to 10 mM , or glycerol. Each column requires a minimum elution volume of 500 mul. Our products are to be used forResearch Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval. Back Purification workflow for Capturem His-Tagged Purification 96-Well Plate.The his-tagged protein of interest is first bound to the membrane, and then washed and eluted with the appropriate buffers. Note: protocol time varies for each format. Check user manuals for specifics on protocol times. Back Available Capturem spin column and filtration device formats.Pictured from left to right: nanoprep, miniprep, 96-well, 24-well, maxiprep, and large-volume Capturem formats. Formats available vary for the different functionalized membranes. Check product pages for list of available formats. Back Load volumes and yields of Capturem purification formats. Back Back Back