CapturemTM Streptavidin 96-Well Plate

Description

The Capturem Streptavidin 96-Well Plate is a single-use, disposable plate for simple, rapid enrichment of target proteins and antibodies that bind biotinylated protein. The plate binds more than 15 mug of biotinylated BSA control per well. This plate is suitable for enrichment of target proteins and antibodies, including those from animal serum, cell culture lysates e.g., mammalian or bacterial cell lysates , and cell culture supernatants of hybridoma cell lines. Each well can hold up to 1 ml of sample and requires a minimum elution volume of 50 mul. Our products are to be used forResearch Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval. Back Available Capturem spin column and filtration device formats.Pictured from left to right: nanoprep, miniprep, 96-well, 24-well, maxiprep, and large-volume Capturem formats. Formats available vary for the different functionalized membranes. Check product pages for list of available formats. Back Load volumes and yields of Capturem purification formats. Back Successive capture of antibodies in triplicate using Capturem Streptavidin. Panels AC.First, 48 mug of biotinylated rabbit IgG in 200 mul Binding Buffer was passed through an equilibrated Capturem Streptavidin spin column, and 32.0 +/- 1.4 mug Panel B was successfully immobilized on the membrane. Following a single wash step, a sample containing the spiked-in target antibody 100 mug of anti-rabbit IgG from goat in hybridoma medium with 20 % mouse serum was diluted with Binding Buffer and applied to the Capturem Streptavidin column. After two successive washing steps with Binding Buffer and then PBS, the enriched target antibody was eluted from the biotinylated capture antibody in 1.0 M glycine in three steps to yield 42 +/- 5 mug Panel C of highly pure target antibody. Back Capturem Streptavidin 96-Well Plate workflow.Note: protocol time varies for each format. Check user manuals for specifics on protocol times. Back Highly reproducible capture in Capturem Streptavidin 96-well plates.Eight technical replicates were loaded with either biotinylated oligonucleotide Panel A or biotinylated BSA Panel B and captured using centrifugation. Bound molecules were quantified by measuring the O.D. of samples before and after loading through the column. Standard protocols with these 96-well plates can be performed in under 15 minutes. Back