CAR Receptor Booster

Description

The CAR Receptor Booster increases the transduction efficiency of target cells to infection by adenovirus. The CAR Receptor Booster consists of exosome-like particles coated with adenoviral receptor protein CAR. When applied to target cells, fusion of the particles with the host cell plasma membrane temporarily increases surface levels of CAR to allow for efficient adenoviral transduction. Additionally, CAR Receptor Booster renders cells that cannot usually be transduced with adenovirus e.g. hematopoietic cells susceptible to infection. Our products are to be used forResearch Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval. Back Human mesenchymal stem cells hMSCs that are normally resistant to transduction by adenovirus vectors are rendered susceptible by treatment with the CAR Receptor Booster.hMSCs were plated in 6 well plates 24 hr prior to transduction. CAR Receptor Booster 20 mul was added, and the cells were incubated for 2 hr at 37C followed by addition of Adeno-XZsGreen1virus MOI190 . Cells were assayed for fluorescence activity 24-hr post transduction. Back Adenoviral transduction of CHO-K1 cells is facilitated by pre-treatment with CAR Receptor Booster.CHO-K1 cells were plated 24 hr prior to transduction and treated for 2 hr with 10 mul of CAR Receptor Booster according to the protocol. The cells were then incubated with Adeno-X LacZ virus MOI=34 for 72 hr and stained for beta galactosidase expression. Only the CHO-K1 cells that were pre-treated with the CAR Receptor Booster were successfully transduced with adenovirus. Back CAR Receptor Boosters are concentrated exosome-like vesicles that are applied to your target cells prior to infection with adenovirus.Boosters result in increased transduction efficiency because treatment increases the cell-surface density of the receptor recognized by the adenovirus. Using this technology, you can now even transduce hematopoietic cell types with adenovirus by simply pre-treating the cells with the CAR Receptor Booster, or you can use the technology to equalize the infection efficiency across multiple target cell types.. Back