
M0386S · 90 pmol
New England Biolabs · Cat: M0386S
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neb.com/en/products/m0386-cas9-nuclease-s-pyogenesDescription
Cas9 Nuclease, S. pyogenes, is an RNA-guided endonuclease that catalyzes site-specific cleavage of double stranded DNA. The location of the break is within the target sequence 3 bases from the NGG PAM Protospacer Adjacent Motif . The PAM sequence, NGG, must follow the targeted region on the opposite strand of the DNA with respect to the region complementary sgRNA sequence.