Chaperone Plasmid Set

Description

The Chaperone Plasmid Set consists of five different plasmids, each of which is designed to express multiple molecular chaperones that function as a chaperone team to enable protein folding. Co-expression of a target protein with one of these chaperone teams increases the recovery of soluble proteins. Each plasmid carries an origin of replication derived from pACYC and a Cmrgene, which allows use withE. coliexpression systems utilizing ColE1-type plasmids containing an ampicillin resistance gene as a marker. The chaperone genes are situated downstream of anaraBorPzt-1 tet promoter. Therefore, expression of target proteins and chaperones can be induced individually if the target gene is placed under the control of other promoters e.g.lac . These plasmids also contain the necessary regulator araCortetr for each promoter. Use this set to perform a two step method to construct target/chaperone co-expression systems: In the first step prepare anE. colihost transformed with only the chaperone plasmid. The second step is to prepare competent cells from this new strain and transform this strain with a plasmid expressing the target protein. NOTE:For commercial use of this product, please refer to theprotein expression vectors for commercial use pagefor ordering information. Our products are to be used forResearch Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval. Back Back Vector maps of Takara Bios chaperone plasmids. Back