ChIP Elute Kit

Description

The ChIP Elute Kit is designed to be used downstream of a chromatin immunoprecipitation ChIP assay to elute DNAassociated with the antibody. The ChIP Elute Kit allows for simultaneous elution and crosslinking reversal of nuclear protein-DNA complexes in a single step. The resulting DNA is single stranded ssDNA and is purified and concentrated with the ChIP-grade reagents provided in the kit. The output from this kit is directly compatible with ChIP-seq library preparation using the DNA SMART ChIP-Seq Kit. The DNA SMART ChIP-Seq Kit generates sequencing libraries compatible with Illumina platforms from single-stranded or double-stranded fragmented DNA. Our products are to be used forResearch Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval. Back Required Products SeqAmp DNA Polymerase is a high-fidelity PCR enzyme with hot start capabilities that is especially well-suited for use with specific SMARTer kits for transcriptome analysis NGS . This optimized PCR enzyme has been shown to perform well even with challenging templates containing GC-rich and AT-rich regions. DNA Polymerase is sold as part of the SMARTer Stranded RNA-Seq Kit. Back cDNA libraries produced using the SMARTer Stranded RNA-Seq Kit show comparable yields and purity irrespective of input RNA concentrations.Agilent 2100 Bioanalyzer gel-like image using a High Sensitivity DNA chip with cDNA libraries produced from between 100 ng100 pg human brain poly A +RNA with the number of PCR cycles indicated. cDNA libraries were produced by amplification with SeqAmp DNA Polymerase included in the SMARTer Stranded RNA-Seq Kit . Data is shown for the SMARTer Stranded RNA-Seq Kit. SMART-Seq Total RNA Mid Input is an equivalent replacement. Back SeqAmp DNA Polymerase is a high fidelity PCR enzyme with hot start capabilities that is especially well-suited for use with specific SMARTer kits for transcriptome analysis NGS . This optimized PCR enzyme has been shown to perform well even with challenging templates containing GC-rich and AT-rich regions. DNA Polymerase is sold as part of the SMARTer Stranded RNA-Seq Kit. Back