circRNA Isolation Kit

Description

abms circRNA Isolation Kit allows for the enrichment of a novel class of RNA called circular RNA from a total RNA sample. Poly A Polymerase, E. coli catalyzes the addition of adenosine residues to the 3 termini of all linear RNAs including structured RNAs. This results in RNAs exhibiting a long, 3 unstructured overhang which is optimally bound by RNase R. RNase R can then illicit 3 to 5 exonuclease activity resulting in improved degradation of linear RNAs which contributes to higher purity circRNAs. Product Component Quantity Poly A Polymerase, E. coli 25 mul 10X Poly A Polymerase, E. coli Reaction Buffer 1.0 ml ATP 10 mM 50 mul RNA Purification Magnetic Beads 5 ml RNase R 50 mul 10X RNase R Reaction Buffer 1.0 ml RNaseOFF Ribonuclease Inhibitor 100 mul