Citrate Antigen Retrieval Buffer (20×, pH 6.0)

Description

After fixation with paraformaldehyde, formalin, or other aldehyde-based fixatives, proteins become cross-linked and carboxymethylated, masking antigenic epitopes and leading to false-negative or weak signals in immunostaining. These cross-links can be reversed under high-temperature or enzymatic conditions, allowing proteins to regain their native conformation. This process—antigen retrieval (AR)—is essential for reliable IHC/IF results. Heat-induced epitope retrieval (HIER) or enzymatic retrieval can be selected according to tissue and antigen characteristics, and a retrieval buffer of appropriate pH should be used.This product is a 20× citrate antigen retrieval buffer (pH 6.0). Dilute 1:20 to obtain the working solution, suitable for heat-mediated antigen retrieval.This production can be used with mIHC,IHC,IF