Clontech PCR-SelectTM Bacterial Genome Subtraction Kit

Description

Complete kit for comparing two bacterial genomes from two different species of bacteria or two different strains of the same species and obtaining DNA fragments that are present in one genome but not in the other. Enough reagents are provided for one control and six complete subtractions. Our products are to be used forResearch Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval. Back Differences in gene content between unrelatedH. pyloristrains.Two unrelatedH. pyloristrains were used for PCR-Select subtraction. J166 was used as tester; 26695 was used as driver. After amplification by PCR, unsubtracted amplified tester Lane 1 and driver Lane 2 samples were electrophoresed on a 1.5 % agarose gel and transferred onto nylon filters. These filters were hybridized with randomly picked clones from the cloned subtracted library Panels AD . Out of 20 clones analyzed, 10 hybridized only to the tester e.g., Panels A & B or hybridized to the tester with higher efficiency e.g., Panel C . Back Required Products This kit is based on Takaras Advantage 2 Polymerase Mix. It contains all reagents needed for a hot-start PCR, including control template and primer mix. This kit is ideal for PCR applications that require high fidelity, such as cDNA amplification or library construction. Enough reagents are supplied for 100 PCR reactions of 50 mul each. Back Back Back Advantage 2 Polymerase Mix is a hot-start PCR polymerase mix that contains Takaras TitaniumTaqDNA Polymerase with TaqStart Antibody , and a minor amount of a proofreading polymerase for a balance of higher yield and higher fidelity than wild-typeTaq. The Mix is ideal for amplification of long templates up to 18 kb and complex genomic DNA up to 6 kb. Two different optimized buffers are supplied with the polymerase mix. dNTPs need to be purchased separately. Enough enzyme mix and PCR buffer are supplied for 100 PCR reactions of 50 mul each. Back Back Back A monoclonal antibody isotype IgG2b that specifically binds to and inactivatesTaqDNA polymerase below 70C for use in hot-start PCR. PCR with TaqStart Antibody has been proven to prevent generation of nonspecific amplification products and primer-dimer artifacts. Back Back Back