Column-Pure Plasmid Miniprep Kit

Description

abms Column-Pure Plasmid Miniprep Kit utilizes the standard alkaline lysis method with silica spin column technology to rapidly extract and purify plasmid DNA from cells. The extracted high quality plasmid DNA is primarily in supercoiled form and is ready for downstream applications such as PCR, restriction digest and sequencing. Principle: The procedure involves an alkaline lysis method to break down bacterial cells, followed by DNA adsorption onto silica in the presence of high salt. The unique silica membrane replaces traditional glass or silica slurries, streamlining the plasmid DNA preparation. The process includes three steps: 1 bacterial lysate preparation and clearing via alkaline lysis, 2 transfer of the supernatant to a column for DNA binding, and 3 washing away proteins and impurities, followed by nucleic acid elution with a low-salt buffer. Key Features: Value-priced 15 min simplified protocol High Capacity - up to 35mug of DNA per column Extracts small & large sized plasmids No phenol chloroform or ethanol precipitation required