CYP3A5 Point Mutation Huh-7 Cell Line

Description

Cells were derived from HuH-7 cells. Cells were then genetically modified using CRISPR/Cas9 method with two guide RNAs to heterozygously delete CYP3A5 3 splice junction. Cells have very high CYP3A5 activity whereas the HuH-7 parental cell line had minimal CYP3A5 activity as assessed by tacrolimus and midazolam metabolism assays with or without CYP3A4 selective inhibitor CYP3CIDE.