C
GENERAL CATALOGUE
CYP3A5 Single Deleted HuH-7 Cell Line
T6347 · 1 x 106 cells / 1.0 mL
abm good · Cat: T6347
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Manufacturer product page
abmgood.com/cyp3a5-single-deleted-huh-7-cell-line.htmlCatalog No: T6347
Category: Cellular Materials
Pack Size: 1 x 106 cells / 1.0 mL
Brand: abm good
Description
Cells were derived from HuH-7 cells. Cells were then genetically modified using CRISPR/Cas9 method with two guide RNAs to heterozygously delete CYP3A5 3 splice junction. Cells have very high CYP3A5 activity whereas the HuH-7 parental cell line had minimal CYP3A5 activity as assessed by tacrolimus and midazolam metabolism assays with or without CYP3A4 selective inhibitor CYP3CIDE.
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