D/D Solubilizer

Description

The D/D Solubilizer is a membrane-permeant ligand that solubilizes complexes formed by proteins fused to the self-assembly domain DmrD , provided in the iDimerize Reverse Dimerization System Cat. No. 635066 . Our products are to be used forResearch Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval. Back Inducible secretion of proteins.Fusion proteins containing DmrD domains localize to the endoplasmic reticulum as massive aggregates left . When the D/D Solubilizer is added, it dissolves the aggregates and allows the protein to be exported through the secretory apparatus right . To ensure secretion of the authentic protein, a furin cleavage site is positioned between the DmrD domains and the protein of interest. Since furin is exclusively expressed in the trans Golgi, the fusion protein will be processed as it traverses this compartment, resulting in the secretion of the correctly processed protein. This method has been used to induce rapid, transient and tightly regulated secretion of human growth hormone hGH and insulin Rivera, V. M.,et al. 2000 Science287 5454 :826830 . Back Secretion of DmrD-tagged luciferase after addition of D/D Solubilizer.7 hr after transfection with a DmrD-taggedMetridialuciferase construct, cells were split into wells of a 6-well plate. The medium was removed and fresh medium was added containing increasing concentrations of D/D Solubilizer. 18 hr later, the media was collected and analyzed using our Ready-To-Glow Secreted Luciferase Reporter System Cat. No. 631731 . Back Back Back