DmrB Monoclonal Antibody

Description

The DmrB Monoclonal Antibody recognizes DmrB-tagged fusion proteins expressed using any iDimerize Inducible Homodimer System. The isotype of this mouse monoclonal antibody is IgG1 Kappa. The antibodies are also able to detect fusions created using ARGENT regulation systems: DmrA is identical to wild type FKBP12, DmrB is identical to the F36V mutant of FKBP12, DmrC is identical to the T82L mutant of FRB. Our products are to be used forResearch Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval. Back Western blot detection of a DmrB-tagged protein using the DmrB Monoclonal Antibody Cat. # 635090 .HEK 293 cells were transfected with the pAcGFP1-DmrB plasmid. Lysate from the equivalent of 3.5 x 104cells was run on a polyacrylamide gel Lanes 2 and 3 and analyzed by Western blot using the DmrB Monoclonal Antibody 1:1000 dilution . A predominant band was detected at the expected molecular weight. The band was detected in lysate from cells treated with Lane 3 or without Lane 2 B/B Homodimerizer, but not in lysate from untransfected HEK 293 cells Lane 1 . Back