DNA SMARTTM ChIP-Seq Kit - 12

Description

ChIP sequencing ChIP-seq combines chromatin immunoprecipitation ChIP with next-generation sequencing NGS to identify the location of protein binding to DNA. The DNA SMART ChIP-Seq Kit generates indexed ChIP-seq libraries suitable for NGS on Illumina sequencing platforms. Library complexity is preserved from as little as 500 pg of double-stranded or single-stranded input DNA. The kit utilizes Clontechs DNA SMART technology; sequencing libraries are generated without the need for enzymatic cleanup or adapter ligations. All of the necessary reagents for producing ChIP-seq libraries are provided in this kit, including primer sets that contain Illumina high-throughput indexes. There are three different versions of the kit depending on the number and specific Illumina indexes desired. For more information on the indexes supplied in each kit visit:www.takarabio.com. Our products are to be used forResearch Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval. Back Back Back Back Back Back Back Back Back Required Products SeqAmp DNA Polymerase is a high-fidelity PCR enzyme with hot start capabilities that is especially well-suited for use with specific SMARTer kits for transcriptome analysis NGS . This optimized PCR enzyme has been shown to perform well even with challenging templates containing GC-rich and AT-rich regions. DNA Polymerase is sold as part of the SMARTer Stranded RNA-Seq Kit. Back cDNA libraries produced using the SMARTer Stranded RNA-Seq Kit show comparable yields and purity irrespective of input RNA concentrations.Agilent 2100 Bioanalyzer gel-like image using a High Sensitivity DNA chip with cDNA libraries produced from between 100 ng100 pg human brain poly A +RNA with the number of PCR cycles indicated. cDNA libraries were produced by amplification with SeqAmp DNA Polymerase included in the SMARTer Stranded RNA-Seq Kit . Data is shown for the SMARTer Stranded RNA-Seq Kit. SMART-Seq Total RNA Mid Input is an equivalent replacement. Back SeqAmp DNA Polymerase is a high fidelity PCR enzyme with hot start capabilities that is especially well-suited for use with specific SMARTer kits for transcriptome analysis NGS . This optimized PCR enzyme has been shown to perform well even with challenging templates containing GC-rich and AT-rich regions. DNA Polymerase is sold as part of the SMARTer Stranded RNA-Seq Kit. Back