E. coli DNA Ligase

Description

E. coli DNA Ligase catalyzes the formation of phosphodiester bonds between double-stranded DNA fragments with 3-OH and 5-phosphate ends, in the presence of NAD+as the cofactor. Unlike T4 DNA Ligase, E. coli DNA Ligase can only ligate DNA fragments with cohesive ends under standard reaction conditions. E. coli DNA Ligase is supplied in 10 mM potassium phosphate pH 7.5 , 50 mM KCl, 1 mM DTT, 1 mM EDTA and 50 % glycerol. Cat. # 2160B contains 5 of Cat. # 2160A. Please refer to Cat. # 2160A for complete product documentation and resources. Our products are to be used forResearch Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.