E. coliHCP ELISA Kit, 2G

Description

This E. coli HCP 2nd Generation ELISA Kit, Item Number F1020, is intended to replace the current E. coli HCP ELISA Kit, Item Number F410. Cygnus has manufactured, characterized, and qualified large pools of the new reagents that will replace the current reagents. The antibodies were generated against and affinity purified using mild lysate from a number of widely used E. coli strains, similarly to the original critical reagents in the F410 Kit. The coverage of the new antibodies to the E. coli HCP antigen was determined to be 87-88 % by Antibody Affinity Extraction with Mass Spectrometry AAE-MS . More importantly, Cygnus Technologies has demonstrated the equivalency of the new antibodies F1022-AF in the E. coli HCP ELISA, 2G F1020 using a panel of E. coli samples from several independent biomanufacturing processes. For BL21 DE3 processes, a BL21 DE3 E. coli ELISA Kit is also now available F1060 . This E. coli HCP ELISA Kit, 2G provides the specificity and sensitivity LOD 0.3 ng/ml, LLOQ 3 ng/ml to detect HCPs with reproducibility that supports regulatory compliance, aiding in optimal purification process development, process control, routine quality control, and product release testing. Please use Sample Diluent, Item No. I028 , with these kits; available separately. Important Change Notification Memo: E. coli HCP ELISA Kit, 2G Updated F410 Memo, 30 Sep 2024: Continued Supply of the Cygnus Technologies E. coli HCP ELISA, Cat# F410 New BL21 DE3 HCP ELISA Kit, Item # F1060, Now Available