Elastic Fiber Staining Kit,E.V.G Method

Description

EnkiLife provide Elastic Fiber Staining Kit,E.V.G Method. Connective tissue, in a narrow sense, is composed of three types of fibers: collagen fibers, reticular fibers, and elastic fibers. Elastic fibers are mainly distributed in the human arterial walls, alveolar walls, and skin. They appear yellow and are highly refractile when fresh. Elastic fiber staining can reveal changes in elastic fibers in skin tissues, such as elastic nevus, granuloma annulare, and scleroderma. It is also used to display and assess lesions of the endocardium and arteries, to observe whether certain pathological changes are accompanied by proliferation or destruction of elastic fibers, and to identify tumor components such as elastic fibromas. After elastic fiber staining, elastic fiber balls within the tumor can be clearly observed. Commonly used elastic fiber staining methods include the Gomori aldehyde-fuchsin method, resorcin-fuchsin method, orcein method, Victoria blue method, and iron hematoxylin method.EnkiLife Elastic Fiber Staining Solution (E.V.G Method) belongs to the iron hematoxylin staining category. The staining principle is based on the fact that elastin and pre-elastin fibers are highly cross-linked via disulfide bonds. After oxidation by iodine, some disulfide bonds are converted into anionic sulfated derivatives, which are strongly basophilic and selectively absorb the basic dye hematoxylin in the staining solution. This product uses the most commonly used Van Gieson (VG) counterstaining method. After staining, thick fibers are deeply stained, while thin fibers are slightly less distinct. Elastic fibers appear black, collagen fibers red, and muscle fibers and erythrocytes yellow, providing a clear contrast.Application: Elastic Fiber,EVG stain